1JPW

Crystal Structure of a Human Tcf-4 / beta-Catenin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	165
Detector technology	IMAGE PLATE
Collection date	2000-11-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	376.530, 92.650, 49.250
Unit cell angles	90.00, 95.98, 90.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.3045
Rwork	0.304
R-free	0.38980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bct
RMSD bond length	0.008
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.123 *
Completeness [%]	88.4 *
Redundancy	2.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	295	PEG 400, MES, ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH7.5
2	1	drop		200 (mM)
3	1	drop	dithiothreitol	5 (mM)
4	1	drop	protein	7.5 (mg/ml)
5	1	reservoir	PEG4000	14 (%(w/v))
6	1	reservoir	MES	100 (mM)	pH5.5
7	1	reservoir	ammonium sulfate	100 (mM)
8	1	reservoir	dithiothreitol	10 (mM)