1GWD

Tri-iodide derivative of hen egg-white lysozyme

Summary for 1GWD

• Entry DOI: 10.2210/pdb1gwd/pdb
• Related: 132L 193L 194L 1A2Y 1AKI 1AT5 1AT6 1AZF 1B0D 1B2K 1BGI 1BHZ 1BVK 1BVX 1BWH 1BWI 1BWJ 1C08 1C10 1DPW 1DPX 1DQJ 1E8L 1F0W 1F10 1F3J 1FDL 1FLQ 1FLU 1FLW 1FLY 1FN5 1G7H 1G7I 1G7J 1G7L 1G7M 1GPQ 1H6M 1H87 1HC0 1HEL 1HEM 1HEN 1HEO 1HEP 1HEQ 1HER 1HEW 1HF4 1HSW 1HSX 1IC4 1IC5 1IC7 1IEE 1IO5 1IOQ 1IOR 1IOS 1IOT 1IR7 1IR8 1IR9 1JA2 1JA4 1JA6 1JA7 1JIS 1JIT 1JIY 1JJ0 1JJ1 1JJ3 1JPO 1JTO 1KIP 1KIQ 1KIR 1KXW 1KXX 1KXY 1LCN 1LKR 1LKS 1LMA 1LPI 1LSA 1LSB 1LSC 1LSD 1LSE 1LSF 1LSG 1LSM 1LSN 1LSY 1LSZ 1LYO 1LYS 1LYZ 1LZ8 1LZ9 1LZA 1LZB 1LZC 1LZD 1LZE 1LZG 1LZH 1LZN 1LZT 1MEL 1MLC 1QIO 1QTK 1RCM 1RFP 1UCO 1UIA 1UIB 1UIC 1UID 1UIE 1UIF 1UIG 1UIH 1XEI 1XEJ 1XEK 2HFM 2IFF 2LYM 2LYO 2LYZ 2LZH 2LZT 3HFL 3HFM 3LYM 3LYO 3LYT 3LYZ 3LZT 4LYM 4LYO 4LYT 4LYZ 4LZT 5LYM 5LYT 5LYZ 6LYT 6LYZ 7LYZ 8LYZ
• Descriptor: LYSOZYME C, IODIDE ION, 1,2-ETHANEDIOL, ... (7 entities in total)
• Functional Keywords: lysozyme, hydrolase, glycosidase, bacteriolytic enzyme, aller
• Biological source: GALLUS GALLUS (CHICKEN)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 15434.97

Authors

Evans, G.,Bricogne, G. (deposition date: 2002-03-14, release date: 2002-06-06, Last modification date: 2017-06-28)

Primary citation

Evans, G.,Bricogne, G.
Triiodide Derivatization and Combinatorial Counter-Ion Replacement: Two Methods for Enhancing Phasing Signal Using Laboratory Cu Kalpha X-Ray Equipment
Acta Crystallogr.,Sect.D, 58:976-, 2002

PubMed Abstract: A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.

PubMed: 12037300
DOI: 10.1107/S0907444902005486

Experimental method

X-RAY DIFFRACTION (1.77 Å)