1E9H

Thr 160 phosphorylated CDK2 - Human cyclin A3 complex with the inhibitor indirubin-5-sulphonate bound

1E9H の概要

• エントリーDOI: 10.2210/pdb1e9h/pdb
• 関連するPDBエントリー: 1AQ1 1B38 1B39 1CKP 1DM2 1E1V 1E1X 1FIN 1HCK 1HCL 1JST 1JSU 1QMZ
• 分子名称: CELL DIVISION PROTEIN KINASE 2, CYCLIN A3, 2',3-DIOXO-1,1',2',3-TETRAHYDRO-2,3'-BIINDOLE-5'-SULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: cell cycle, complex (protein kinase-cyclin), kinases, inhibitor, cyclin, complex, phosphorylation
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: P20248
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 128360.47

構造登録者

Davies, T.G.,Tunnah, P.,Noble, M.E.M.,Endicott, J.A. (登録日: 2000-10-16, 公開日: 2001-10-11, 最終更新日: 2024-05-01)

主引用文献

Davies, T.G.,Tunnah, P.,Meijer, L.,Marko, D.,Eisenbrand, G.,Endicott, J.A.,Noble, M.E.
Inhibitor Binding to Active and Inactive Cdk2: The Crystal Structure of Cdk2-Cyclin A/Indirubin-5-Sulphonate
Structure, 9:389-, 2001

PubMed Abstract: Cyclin-dependent kinase 2 (CDK2) is an important target for structure-based design of antitumor agents. Monomeric CDK2 is inactive. Activation requires rearrangements to key structural elements of the enzyme's active site, which accompany cyclin binding and phosphorylation. To assess the validity of using monomeric CDK2 as a model for the active kinase in structure-based drug design, we have solved the structure of the inhibitor indirubin-5-sulphonate (E226) complexed with phospho-CDK2-cyclin A and compared it with the structure of E226 bound to inactive, monomeric CDK2.

PubMed: 11377199
DOI: 10.1016/S0969-2126(01)00598-6

実験手法

X-RAY DIFFRACTION (2.5 Å)