8OE4
Cryo-EM structure of a pre-dimerized human IL-23 complete extracellular signaling complex.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Interleukin-12 subunit beta | polymer | 306 | 34740.9 | 1 | UniProt (P29460) Pfam (PF10420) | Homo sapiens (human) | IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40,NK cell stimulatory factor chain 2,NKSF2 |
| 2 | B (B) | Interleukin-23 subunit alpha | polymer | 186 | 20650.1 | 1 | UniProt (Q9NPF7) Pfam (PF16649) | Homo sapiens (human) | IL-23 subunit alpha,IL-23-A,Interleukin-23 subunit p19,IL-23p19 |
| 3 | C (C) | Interleukin-23 receptor,Calmodulin-1 | polymer | 485 | 54991.4 | 1 | UniProt (Q5VWK5) UniProt (P0DP23) Pfam (PF13499) | Homo sapiens (human) | IL-23 receptor,IL-23R |
| 4 | D (D) | Interleukin-12 receptor subunit beta-1,Death-associated protein kinase 1 | polymer | 547 | 60187.3 | 1 | UniProt (P42701) UniProt (P53355) Pfam (PF00041) | Homo sapiens (human) | IL-12 receptor subunit beta-1,IL-12R subunit beta-1,IL-12R-beta-1,IL-12RB1,IL-12 receptor beta component,DAP kinase 1 |
| 5 | E (E) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 6 | F (F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 7 | G, H (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) |
Sequence modifications
A: 23 - 328 (UniProt: P29460)
B: 20 - 189 (UniProt: Q9NPF7)
C: 24 - 353 (UniProt: Q5VWK5)
C: 364 - 508 (UniProt: P0DP23)
D: 24 - 540 (UniProt: P42701)
D: 552 - 570 (UniProt: P53355)
| PDB | External Database | Details |
|---|---|---|
| Asp 303 | Asn 303 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 190 | - | expression tag |
| Asp 191 | - | expression tag |
| Glu 192 | - | expression tag |
| Val 193 | - | expression tag |
| Asp 194 | - | expression tag |
| Gly 195 | - | expression tag |
| His 196 | - | expression tag |
| His 197 | - | expression tag |
| His 198 | - | expression tag |
| His 199 | - | expression tag |
| His 200 | - | expression tag |
| His 201 | - | expression tag |
| His 202 | - | expression tag |
| His 203 | - | expression tag |
| His 204 | - | expression tag |
| His 205 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 354 | - | linker |
| Thr 355 | - | linker |
| Gly 356 | - | linker |
| Gly 357 | - | linker |
| Ser 358 | - | linker |
| Gly 359 | - | linker |
| Gly 360 | - | linker |
| Ser 361 | - | linker |
| Gly 362 | - | linker |
| Gly 363 | - | linker |
D: 24 - 540 (UniProt: P42701)
| PDB | External Database | Details |
|---|---|---|
| Gly 541 | - | linker |
| Thr 542 | - | linker |
| Gly 543 | - | linker |
| Gly 544 | - | linker |
| Ser 545 | - | linker |
| Gly 546 | - | linker |
| Gly 547 | - | linker |
| Ser 548 | - | linker |
| Gly 549 | - | linker |
| Gly 550 | - | linker |
| Ala 551 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 170569.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1173.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.4 | |
| All* | Total formula weight | 172185.3 |
