8FRJ

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SGC0946

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transcription factor ETV6,Guanine-N7 methyltransferase nsp14 chimera	polymer	309	35439.3	1	UniProt (P41212) UniProt (P0DTD1) Pfam (PF02198) Pfam (PF06471)	Severe acute respiratory syndrome coronavirus 2	ETS translocation variant 6,ETS-related protein Tel1,Tel,Non-structural protein 14,nsp14,Proofreading exoribonuclease nsp14,ExoN
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C (A)	5-bromo-7-{5-[(3-{[(4-tert-butylphenyl)carbamoyl]amino}propyl)(propan-2-yl)amino]-5-deoxy-beta-D-ribofuranosyl}-7H-pyrrolo[2,3-d]pyrimidin-4-amine	non-polymer		618.6	1	Chemie (AW2)
4	D (A)	water	water		18.0	386	Chemie (HOH)

Sequence modifications

A: 2 - 78 (UniProt: P41212)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 4	Arg 49	engineered mutation
Glu 67	Val 112	engineered mutation
Ala 77	Lys 122	engineered mutation
Pro 79	-	linker
Ala 80	-	linker
Ala 81	-	linker

A: 300 - 508 (UniProt: P0DTD1)

PDB	External Database	Details
Trp 509	-	expression tag
Val 510	-	expression tag
Tyr 511	-	expression tag
Lys 512	-	expression tag
Gln 513	-	expression tag
Phe 514	-	expression tag
Asp 515	-	expression tag
Thr 516	-	expression tag
Tyr 517	-	expression tag
Asn 518	-	expression tag
Leu 519	-	expression tag
Trp 520	-	expression tag
Asn 521	-	expression tag
Thr 522	-	expression tag
Phe 523	-	expression tag
Thr 524	-	expression tag
Arg 525	-	expression tag
Leu 526	-	expression tag
Gln 527	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35439.3
Non-Polymers*	Number of molecules	2
	Total formula weight	684.0
All*	Total formula weight	36123.2

*Water molecules are not included.