8FRJ

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SGC0946

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	P 65
Unit cell lengths	109.155, 109.155, 48.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.310 - 1.570
R-factor	0.1715
Rwork	0.170
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.934
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.595	1.711
High resolution limit [Å]	1.570	1.570
Rmerge	0.105	1.116
Rmeas	0.116
Rpim	0.050	0.602
Number of reflections	31718	1588
<I/σ(I)>	10.7	1.6
Completeness [%]	94.3
Redundancy	10.2
CC(1/2)	0.997	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate and 0.1M Sodium Citrate pH 5.5