8FFE

Crystal structure of LRP6 E1E2 domains bound to YW210.09 Fab and engineered XWnt8 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Low-density lipoprotein receptor-related protein 6	polymer	613	69302.1	1	UniProt (O75581) Pfam (PF00058) Pfam (PF14670)	Homo sapiens (human)	LRP-6
2	B (H)	YW210.09 Fab heavy chain with engineered XWnt8 NC peptide and linker	polymer	276	28657.8	1		Homo sapiens
3	C (L)	YW210.09 Fab light chain	polymer	225	24426.0	1		Homo sapiens
4	D (B)	alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		716.7	1	In PDB GlyTouCan (G63564LA)
5	E (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		878.8	1	In PDB GlyTouCan (G92953ND)
6	F (D)	alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB
7	G, H, I, J, N... (A, H, L)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
8	K, L, M (A)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
9	Q (L)	SUCCINIC ACID	non-polymer		118.1	1	Chemie (SIN)
10	R, S, T (A, H, L)	water	water		18.0	592	Chemie (HOH)

Sequence modifications

A: 20 - 631 (UniProt: O75581)

PDB	External Database	Details
His 632	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	122386.0
Branched	Number of molecules	3
	Total formula weight	2182.0
Non-Polymers*	Number of molecules	11
	Total formula weight	831.7
All*	Total formula weight	125399.7

*Water molecules are not included.