8FFE
Crystal structure of LRP6 E1E2 domains bound to YW210.09 Fab and engineered XWnt8 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-14 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.212, 91.385, 104.055 |
Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
Resolution | 44.370 - 1.720 |
R-factor | 0.1873 |
Rwork | 0.185 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.974 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.370 | 1.781 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmeas | 0.104 | 2.243 |
Rpim | 0.039 | 0.856 |
Number of reflections | 109415 | 6719 |
<I/σ(I)> | 10.49 | 0.64 |
Completeness [%] | 90.6 | 55.47 |
Redundancy | 7.1 | 6.7 |
CC(1/2) | 0.997 | 0.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM SPG (succinate/phosphate/glycine buffer), 25% PEG 1500 |