8E99

Human GluN1a-GluN2A-GluN2C triheteromeric NMDA receptor in complex with Nb-4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glutamate receptor ionotropic, NMDA 1	polymer	847	95081.7	2	UniProt (Q05586) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Homo sapiens (human)	GluN1,Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1,NMD-R1
2	B (B)	Glutamate receptor ionotropic, NMDA 2A	polymer	875	97770.9	1	UniProt (Q12879) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Homo sapiens (human)	GluN2A,Glutamate [NMDA] receptor subunit epsilon-1,N-methyl D-aspartate receptor subtype 2A,NMDAR2A,NR2A,hNR2A
3	D (D)	Glutamate receptor ionotropic, NMDA 2C	polymer	880	96958.4	1	UniProt (Q14957) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Homo sapiens (human)	GluN2C,Glutamate [NMDA] receptor subunit epsilon-3,N-methyl D-aspartate receptor subtype 2C,NMDAR2C,NR2C
4	E (E)	Nanobody 4	polymer	163	16549.5	1		Lama glama (llama)
5	F, G, H, I (F, G, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
6	J, K (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A, C: 1 - 843 (UniProt: Q05586)

PDB	External Database	Details
His 5	Arg 5	conflict
Phe 9	Leu 9	conflict
Phe 17	Val 17	conflict
Ser 22	Cys 22	conflict
Asn 844	-	expression tag
Gly 845	-	expression tag
Ala 846	-	expression tag
Gln 847	-	expression tag

B: 30 - 851 (UniProt: Q12879)

PDB	External Database	Details
Gly -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Ala -11	-	expression tag
Thr -10	-	expression tag
Asn -9	-	expression tag
Phe -8	-	expression tag
Ser -7	-	expression tag
Leu -6	-	expression tag
Leu -5	-	expression tag
Lys -4	-	expression tag
Gln -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Asp 0	-	expression tag
Val 1	-	expression tag
Glu 2	-	expression tag
Glu 3	-	expression tag
Asn 4	-	expression tag
Pro 5	-	expression tag
Gly 6	-	expression tag
Pro 7	-	expression tag
Met 8	-	expression tag
Gly 9	-	expression tag
Thr 10	-	expression tag
Met 11	-	expression tag
Arg 12	-	expression tag
Leu 13	-	expression tag
Phe 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Ala 17	-	expression tag
Val 18	-	expression tag
Leu 19	-	expression tag
Phe 20	-	expression tag
Leu 21	-	expression tag
Phe 22	-	expression tag
Ser 23	-	expression tag
Phe 24	-	expression tag
Ala 25	-	expression tag
Arg 26	-	expression tag
Ala 27	-	expression tag
Thr 28	-	expression tag
Gly 29	-	expression tag
Ser 231	Cys 231	conflict
Ser 399	Cys 399	conflict
Ser 460	Cys 460	conflict
Ser 848	Cys 848	conflict
Thr 852	-	expression tag
Glu 853	-	expression tag
Thr 854	-	expression tag
Ser 855	-	expression tag
Gln 856	-	expression tag
Val 857	-	expression tag
Ala 858	-	expression tag
Pro 859	-	expression tag
Ala 860	-	expression tag

D: 26 - 849 (UniProt: Q14957)

PDB	External Database	Details
Met -30	-	expression tag
Gly -29	-	expression tag
Thr -28	-	expression tag
Met -27	-	expression tag
Arg -26	-	expression tag
Leu -25	-	expression tag
Phe -24	-	expression tag
Leu -23	-	expression tag
Leu -22	-	expression tag
Ala -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Leu -17	-	expression tag
Phe -16	-	expression tag
Ser -15	-	expression tag
Phe -14	-	expression tag
Ala -13	-	expression tag
Arg -12	-	expression tag
Ala -11	-	expression tag
Thr -10	-	expression tag
Gly -9	-	expression tag
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Ser 10	-	expression tag
Ala 11	-	expression tag
Trp 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
Pro 15	-	expression tag
Gln 16	-	expression tag
Phe 17	-	expression tag
Glu 18	-	expression tag
Lys 19	-	expression tag
Gly 20	-	expression tag
Ala 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	401442.2
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	403582.2

*Water molecules are not included.