8DCS

Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (cattle)	Transducin beta chain 1
2	B (N)	Nanobody 35	polymer	140	15282.9	1		Lama glama
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	384	44694.6	1	UniProt (P04896) Pfam (PF00503)	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
5	E (R)	Endolysin,Endolysin,Beta-1 adrenergic receptor chimera	polymer	508	57958.7	1	UniProt (P00720) Pfam (PF00959) Pfam (PF00001) UniProt (by SIFTS) (P07700)	Meleagris gallopavo (turkey)	Lysis protein,Lysozyme,Muramidase
6	F (R)	4-{[(2S)-3-(tert-butylamino)-2-hydroxypropyl]oxy}-3H-indole-2-carbonitrile	non-polymer		287.4	1	Chemie (P32)

Sequence modifications

A: 1 - 380 (UniProt: P04896)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Leu -1	-	expression tag
Ala 0	-	expression tag
Gly 18	Ala 18	conflict
Ser 72	Gly 72	conflict

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: -130 - 29 (UniProt: P00720)

PDB	External Database	Details
Met -162	-	initiating methionine
Lys -161	-	expression tag
Thr -160	-	expression tag
Ile -159	-	expression tag
Ile -158	-	expression tag
Ala -157	-	expression tag
Leu -156	-	expression tag
Ser -155	-	expression tag
Tyr -154	-	expression tag
Ile -153	-	expression tag
Phe -152	-	expression tag
Cys -151	-	expression tag
Leu -150	-	expression tag
Val -149	-	expression tag
Phe -148	-	expression tag
Ala -147	-	expression tag
Asp -146	-	expression tag
Tyr -145	-	expression tag
Lys -144	-	expression tag
Asp -143	-	expression tag
Asp -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Lys -139	-	expression tag
Leu -138	-	expression tag
Glu -137	-	expression tag
Val -136	-	expression tag
Leu -135	-	expression tag
Phe -134	-	expression tag
Gln -133	-	expression tag
Gly -132	-	expression tag
Pro -131	-	expression tag
Gly -120	Arg 12	conflict
Thr -78	Cys 54	conflict
Ala -35	Cys 97	conflict
Arg 5	Ile 137	conflict
Ala 30	-	linker
Ala 31	-	linker
Gly 32	-	linker

R: 33 - 380 (PDB: 8DCS)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	163066.9
Non-Polymers*	Number of molecules	1
	Total formula weight	287.4
All*	Total formula weight	163354.3

*Water molecules are not included.