8DCS
Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62871) Pfam (PF00400) | Bos taurus (cattle) | Transducin beta chain 1 |
| 2 | B (N) | Nanobody 35 | polymer | 140 | 15282.9 | 1 | Lama glama | ||
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 384 | 44694.6 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (cattle) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 5 | E (R) | Endolysin,Endolysin,Beta-1 adrenergic receptor chimera | polymer | 508 | 57958.7 | 1 | UniProt (P00720) Pfam (PF00959) Pfam (PF00001) UniProt (by SIFTS) (P07700) | Meleagris gallopavo (turkey) | Lysis protein,Lysozyme,Muramidase |
| 6 | F (R) | Cyanopindolol | non-polymer | 287.4 | 1 | Chemie (P32) |
Sequence modifications
A: 1 - 380 (UniProt: P04896)
G: 1 - 71 (UniProt: P63212)
R: -130 - 29 (UniProt: P00720)
R: 33 - 380 (PDB: 8DCS)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Gly 18 | Ala 18 | conflict |
| Ser 72 | Gly 72 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | Cys 68 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -162 | - | initiating methionine |
| Lys -161 | - | expression tag |
| Thr -160 | - | expression tag |
| Ile -159 | - | expression tag |
| Ile -158 | - | expression tag |
| Ala -157 | - | expression tag |
| Leu -156 | - | expression tag |
| Ser -155 | - | expression tag |
| Tyr -154 | - | expression tag |
| Ile -153 | - | expression tag |
| Phe -152 | - | expression tag |
| Cys -151 | - | expression tag |
| Leu -150 | - | expression tag |
| Val -149 | - | expression tag |
| Phe -148 | - | expression tag |
| Ala -147 | - | expression tag |
| Asp -146 | - | expression tag |
| Tyr -145 | - | expression tag |
| Lys -144 | - | expression tag |
| Asp -143 | - | expression tag |
| Asp -142 | - | expression tag |
| Asp -141 | - | expression tag |
| Asp -140 | - | expression tag |
| Lys -139 | - | expression tag |
| Leu -138 | - | expression tag |
| Glu -137 | - | expression tag |
| Val -136 | - | expression tag |
| Leu -135 | - | expression tag |
| Phe -134 | - | expression tag |
| Gln -133 | - | expression tag |
| Gly -132 | - | expression tag |
| Pro -131 | - | expression tag |
| Gly -120 | Arg 12 | conflict |
| Thr -78 | Cys 54 | conflict |
| Ala -35 | Cys 97 | conflict |
| Arg 5 | Ile 137 | conflict |
| Ala 30 | - | linker |
| Ala 31 | - | linker |
| Gly 32 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 163066.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 287.4 | |
| All* | Total formula weight | 163354.3 |
