8DCS
Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0005159 | molecular_function | insulin-like growth factor receptor binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005834 | cellular_component | heterotrimeric G-protein complex |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| A | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| A | 0007606 | biological_process | sensory perception of chemical stimulus |
| A | 0010856 | molecular_function | adenylate cyclase activator activity |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| A | 0031698 | molecular_function | beta-2 adrenergic receptor binding |
| A | 0031748 | molecular_function | D1 dopamine receptor binding |
| A | 0031852 | molecular_function | mu-type opioid receptor binding |
| A | 0035255 | molecular_function | ionotropic glutamate receptor binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051430 | molecular_function | corticotropin-releasing hormone receptor 1 binding |
| A | 0071880 | biological_process | adenylate cyclase-activating adrenergic receptor signaling pathway |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005834 | cellular_component | heterotrimeric G-protein complex |
| B | 0007165 | biological_process | signal transduction |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| B | 0016020 | cellular_component | membrane |
| B | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| B | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| B | 0071870 | biological_process | cellular response to catecholamine stimulus |
| B | 0097381 | cellular_component | photoreceptor disc membrane |
| G | 0003924 | molecular_function | GTPase activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005834 | cellular_component | heterotrimeric G-protein complex |
| G | 0005886 | cellular_component | plasma membrane |
| G | 0007165 | biological_process | signal transduction |
| G | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| G | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| G | 0016020 | cellular_component | membrane |
| G | 0031681 | molecular_function | G-protein beta-subunit binding |
| G | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| G | 0071870 | biological_process | cellular response to catecholamine stimulus |
| R | 0003796 | molecular_function | lysozyme activity |
| R | 0004930 | molecular_function | G protein-coupled receptor activity |
| R | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| R | 0009253 | biological_process | peptidoglycan catabolic process |
| R | 0016020 | cellular_component | membrane |
| R | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| R | 0016998 | biological_process | cell wall macromolecule catabolic process |
| R | 0030430 | cellular_component | host cell cytoplasm |
| R | 0031640 | biological_process | killing of cells of another organism |
| R | 0042742 | biological_process | defense response to bacterium |
| R | 0044659 | biological_process | viral release from host cell by cytolysis |
Functional Information from PROSITE/UniProt
| site_id | PS00237 |
| Number of Residues | 17 |
| Details | G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASIeTLCVIAIDRYLaI |
| Chain | Residue | Details |
| R | ALA127-ILE143 |
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
| Chain | Residue | Details |
| B | LEU70-SER84 | |
| B | ILE157-ILE171 | |
| B | LEU285-ALA299 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | GLU-121 | |
| A | LYS197 | |
| A | CYS223 | |
| A | SER292 | |
| A | ARG366 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_04110, ECO:0000269|PubMed:1892846, ECO:0000269|PubMed:3382407, ECO:0000269|PubMed:7831309, ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | ASP-112 | |
| A | ASN204 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8266098 |
| Chain | Residue | Details |
| R | LEU-100 | |
| R | PHE-28 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000303|PubMed:7831309 |
| Chain | Residue | Details |
| R | SER-15 | |
| R | ASN0 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=1 |
| Chain | Residue | Details |
| R | GLN39-GLY67 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 78 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:18594507 |
| Chain | Residue | Details |
| R | SER68-THR76 | |
| R | ASP138-ARG155 | |
| R | ARG232-VAL320 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 26 |
| Details | TRANSMEM: Helical; Name=2 |
| Chain | Residue | Details |
| R | ASN77-VAL103 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 40 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:18594507 |
| Chain | Residue | Details |
| R | ARG104-GLU115 | |
| R | HIS180-ARG205 | |
| R | LYS351-ARG355 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 21 |
| Details | TRANSMEM: Helical; Name=3 |
| Chain | Residue | Details |
| R | LEU116-ILE137 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 23 |
| Details | TRANSMEM: Helical; Name=4 |
| Chain | Residue | Details |
| R | ALA156-MET179 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=5 |
| Chain | Residue | Details |
| R | ALA206-TYR231 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 29 |
| Details | TRANSMEM: Helical; Name=6 |
| Chain | Residue | Details |
| R | PRO321-ARG350 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=7 |
| Chain | Residue | Details |
| R | LEU356-HIS378 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18594507, ECO:0007744|PDB:2VT4 |
| Chain | Residue | Details |
| R | ASP121 | |
| R | SER211 | |
| R | ASP364 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000250 |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 921 |
| Chain | Residue | Details |
| R | GLU-121 | proton shuttle (general acid/base) |
| R | ASP-112 | covalent catalysis |
