7ZCW
Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 2 | UniProt (P68363) Pfam (PF00091) Pfam (PF03953) In PDB | Homo sapiens (human) | Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain |
2 | B, F, G, H | Tubulin beta-2B chain | polymer | 445 | 49907.8 | 4 | UniProt (Q9BVA1) Pfam (PF00091) Pfam (PF03953) In PDB | Homo sapiens (human) | |
3 | C | Tubulinyl-Tyr carboxypeptidase 2 | polymer | 355 | 40488.9 | 1 | UniProt (Q86V25) Pfam (PF14822) In PDB | Homo sapiens (human) | Vasohibin-2,Vasohibin-like protein |
4 | D | Small vasohibin-binding protein | polymer | 73 | 8780.0 | 1 | UniProt (Q8N300) Pfam (PF15674) In PDB | Homo sapiens (human) | Coiled coil domain-containing protein 23 |
5 | A, E | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
6 | A, B, E, F, G... | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
7 | B, F, G, H | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | non-polymer | 521.2 | 4 | Chemie (G2P) |
Sequence modifications
B, F, G, H: 1 - 445 (UniProt: Q9BVA1)
C: 1 - 355 (UniProt: Q86V25)
D: 1 - 66 (UniProt: Q8N300)
PDB | External Database | Details |
---|---|---|
Ala 55 | Thr 55 | conflict |
Val 170 | Met 170 | conflict |
Ala 296 | Ser 296 | conflict |
Val 316 | Ile 316 | conflict |
PDB | External Database | Details |
---|---|---|
Ala 158 | Cys 158 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Lys 67 | - | expression tag |
His 68 | - | expression tag |
His 69 | - | expression tag |
His 70 | - | expression tag |
His 71 | - | expression tag |
His 72 | - | expression tag |
His 73 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 349308.9 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 3277.0 | |
All* | Total formula weight | 352585.9 |