7ZCW

Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P68363) Pfam (PF00091) Pfam (PF03953)	Homo sapiens (human)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, F, G, H (B, F, G, H)	Tubulin beta-2B chain	polymer	445	49907.8	4	UniProt (Q9BVA1) Pfam (PF00091) Pfam (PF03953)	Homo sapiens (human)
3	C (C)	Tubulinyl-Tyr carboxypeptidase 2	polymer	355	40488.9	1	UniProt (Q86V25) Pfam (PF14822)	Homo sapiens (human)	Vasohibin-2,Vasohibin-like protein
4	D (D)	Small vasohibin-binding protein	polymer	73	8780.0	1	UniProt (Q8N300) Pfam (PF15674)	Homo sapiens (human)	Coiled coil domain-containing protein 23
5	I, M (A, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	J, L, N, P, R... (A, B, E, F, G...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	K, O, Q, S (B, F, G, H)	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	4	Chemie (G2P)

Sequence modifications

B, F, G, H: 1 - 445 (UniProt: Q9BVA1)

PDB	External Database	Details
Ala 55	Thr 55	conflict
Val 170	Met 170	conflict
Ala 296	Ser 296	conflict
Val 316	Ile 316	conflict

C: 1 - 355 (UniProt: Q86V25)

PDB	External Database	Details
Ala 158	Cys 158	engineered mutation

D: 1 - 66 (UniProt: Q8N300)

PDB	External Database	Details
Lys 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag
His 71	-	expression tag
His 72	-	expression tag
His 73	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	349308.9
Non-Polymers*	Number of molecules	12
	Total formula weight	3277.0
All*	Total formula weight	352585.9

*Water molecules are not included.