7ZCW
Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 153041 |
| Software | RELION,PHENIX,Coot,SerialEM,Gctf,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
