7ZCW

Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003725	molecular_function	double-stranded RNA binding
A	0003924	molecular_function	GTPase activity
A	0005198	molecular_function	structural molecule activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0005881	cellular_component	cytoplasmic microtubule
A	0007017	biological_process	microtubule-based process
A	0015630	cellular_component	microtubule cytoskeleton
A	0016787	molecular_function	hydrolase activity
A	0030705	biological_process	cytoskeleton-dependent intracellular transport
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0051301	biological_process	cell division
A	0071353	biological_process	cellular response to interleukin-4
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0001764	biological_process	neuron migration
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0007399	biological_process	nervous system development
B	0015630	cellular_component	microtubule cytoskeleton
B	0021987	biological_process	cerebral cortex development
B	0045171	cellular_component	intercellular bridge
B	0046872	molecular_function	metal ion binding
B	0046982	molecular_function	protein heterodimerization activity
B	0050804	biological_process	modulation of chemical synaptic transmission
B	0072686	cellular_component	mitotic spindle
B	0098685	cellular_component	Schaffer collateral - CA1 synapse
B	1902669	biological_process	positive regulation of axon guidance
B	1990403	biological_process	embryonic brain development
C	0000768	biological_process	syncytium formation by plasma membrane fusion
C	0001938	biological_process	positive regulation of endothelial cell proliferation
C	0003779	molecular_function	actin binding
C	0004180	molecular_function	carboxypeptidase activity
C	0004181	molecular_function	metallocarboxypeptidase activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0006508	biological_process	proteolysis
C	0008017	molecular_function	microtubule binding
C	0045765	biological_process	regulation of angiogenesis
C	0045766	biological_process	positive regulation of angiogenesis
C	0060674	biological_process	placenta blood vessel development
C	0060711	biological_process	labyrinthine layer development
C	0060716	biological_process	labyrinthine layer blood vessel development
C	0061564	biological_process	axon development
C	0106423	molecular_function	tubulin-tyrosine carboxypeptidase
C	0140253	biological_process	cell-cell fusion
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0006508	biological_process	proteolysis
D	0008017	molecular_function	microtubule binding
D	0009306	biological_process	protein secretion
D	0010596	biological_process	negative regulation of endothelial cell migration
D	0016504	molecular_function	peptidase activator activity
D	0031397	biological_process	negative regulation of protein ubiquitination
D	0045177	cellular_component	apical part of cell
D	0061564	biological_process	axon development
D	1905048	biological_process	regulation of metallopeptidase activity
E	0000226	biological_process	microtubule cytoskeleton organization
E	0000278	biological_process	mitotic cell cycle
E	0003725	molecular_function	double-stranded RNA binding
E	0003924	molecular_function	GTPase activity
E	0005198	molecular_function	structural molecule activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0005881	cellular_component	cytoplasmic microtubule
E	0007017	biological_process	microtubule-based process
E	0015630	cellular_component	microtubule cytoskeleton
E	0016787	molecular_function	hydrolase activity
E	0030705	biological_process	cytoskeleton-dependent intracellular transport
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0051301	biological_process	cell division
E	0071353	biological_process	cellular response to interleukin-4
F	0000226	biological_process	microtubule cytoskeleton organization
F	0000278	biological_process	mitotic cell cycle
F	0001764	biological_process	neuron migration
F	0003924	molecular_function	GTPase activity
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0007017	biological_process	microtubule-based process
F	0007399	biological_process	nervous system development
F	0015630	cellular_component	microtubule cytoskeleton
F	0021987	biological_process	cerebral cortex development
F	0045171	cellular_component	intercellular bridge
F	0046872	molecular_function	metal ion binding
F	0046982	molecular_function	protein heterodimerization activity
F	0050804	biological_process	modulation of chemical synaptic transmission
F	0072686	cellular_component	mitotic spindle
F	0098685	cellular_component	Schaffer collateral - CA1 synapse
F	1902669	biological_process	positive regulation of axon guidance
F	1990403	biological_process	embryonic brain development
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0001764	biological_process	neuron migration
G	0003924	molecular_function	GTPase activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0007017	biological_process	microtubule-based process
G	0007399	biological_process	nervous system development
G	0015630	cellular_component	microtubule cytoskeleton
G	0021987	biological_process	cerebral cortex development
G	0045171	cellular_component	intercellular bridge
G	0046872	molecular_function	metal ion binding
G	0046982	molecular_function	protein heterodimerization activity
G	0050804	biological_process	modulation of chemical synaptic transmission
G	0072686	cellular_component	mitotic spindle
G	0098685	cellular_component	Schaffer collateral - CA1 synapse
G	1902669	biological_process	positive regulation of axon guidance
G	1990403	biological_process	embryonic brain development
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0001764	biological_process	neuron migration
H	0003924	molecular_function	GTPase activity
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005515	molecular_function	protein binding
H	0005525	molecular_function	GTP binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0007399	biological_process	nervous system development
H	0015630	cellular_component	microtubule cytoskeleton
H	0021987	biological_process	cerebral cortex development
H	0045171	cellular_component	intercellular bridge
H	0046872	molecular_function	metal ion binding
H	0046982	molecular_function	protein heterodimerization activity
H	0050804	biological_process	modulation of chemical synaptic transmission
H	0072686	cellular_component	mitotic spindle
H	0098685	cellular_component	Schaffer collateral - CA1 synapse
H	1902669	biological_process	positive regulation of axon guidance
H	1990403	biological_process	embryonic brain development

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
B	GLY140-GLY146
A	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:29146869, ECO:0000305|PubMed:26794318

Chain	Residue	Details
C	ALA158
B	SER138
B	GLY142
B	THR143
B	GLY144
B	ASN204
B	ASN226
F	GLN11
F	SER138
F	GLY142
F	THR143
F	GLY144
F	ASN204
F	ASN226
G	GLN11
G	SER138
G	GLY142
G	THR143
G	GLY144
G	ASN204
G	ASN226
H	GLN11
H	SER138
H	GLY142
H	THR143
H	GLY144
H	ASN204
H	ASN226

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:26794318

Chain	Residue	Details
C	HIS193
C	SER210
G	GLU69
H	GLU69
A	THR145
A	THR179
A	ASN206
A	ASN228
E	GLN11
E	GLU71
E	SER140
E	GLY144
E	THR145
E	THR179
E	ASN206
E	ASN228

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692

Chain	Residue	Details
C	SER302
F	SER40
G	SER40
H	SER40

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q3KRE8

Chain	Residue	Details
B	ALA55
F	ALA55
G	ALA55
H	ALA55

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
B	LYS58
F	LYS58
G	LYS58
H	LYS58

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000269|PubMed:16371510

Chain	Residue	Details
B	SER172
F	SER172
G	SER172
H	SER172

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	THR285
B	THR290
F	THR285
F	THR290
G	THR285
G	THR290
H	THR285
H	THR290

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	ARG318
F	ARG318
G	ARG318
H	ARG318

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:Q2T9S0

Chain	Residue	Details
B	GLU438
F	GLU438
G	GLU438
H	GLU438

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
B	LYS58
F	LYS58
G	LYS58
H	LYS58

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
A	TYR451
B	LYS324
F	LYS324
G	LYS324
H	LYS324

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
A	LYS326
A	LYS370
E	LYS326
E	LYS370

---