7Z13

S. cerevisiae CMGE dimer nucleating origin DNA melting

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, W (2, a)	DNA replication licensing factor MCM2	polymer	868	98911.5	2	UniProt (A0A6A5Q1S9)	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 2
2	B, X (3, b)	DNA replication licensing factor MCM3	polymer	1006	111720.2	2	UniProt (P24279) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF22207)	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 3
3	C, Y (4, c)	DNA replication licensing factor MCM4	polymer	933	105138.4	2	UniProt (P30665) Pfam (PF21128) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (baker's yeast)	Cell division control protein 54
4	D, Z (5, d)	DNA helicase	polymer	775	86505.7	2	UniProt (A0A6A5PUY8)	Saccharomyces cerevisiae (baker's yeast)
5	AA, E (6, e)	DNA replication licensing factor MCM6	polymer	1017	113110.2	2	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 6
6	BA, F (7, f)	DNA replication licensing factor MCM7	polymer	845	95049.9	2	UniProt (A0A6A5Q4N0)	Saccharomyces cerevisiae (baker's yeast)
7	G (A)	DNA (53-MER)	polymer	53	16320.6	1		DNA molecule
8	H (B)	DNA (53-MER)	polymer	53	16311.6	1		DNA molecule
9	I, O (C, J)	DNA replication complex GINS protein PSF3	polymer	229	25718.1	2	UniProt (Q12146) Pfam (PF22466) Pfam (PF05916)	Saccharomyces cerevisiae (baker's yeast)	Partner of Sld five 3
10	J, P (D, K)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	2	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922)	Saccharomyces cerevisiae (baker's yeast)
11	K, Q (E, L)	Cell division control protein 45	polymer	657	75154.7	2	UniProt (Q08032) Pfam (PF02724)	Saccharomyces cerevisiae (baker's yeast)
12	L, R (F, M)	DNA polymerase epsilon subunit B	polymer	689	78425.9	2	UniProt (P24482) Pfam (PF04042)	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase II subunit 2
13	M, T (H, O)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	2	UniProt (A0A6A5Q203)	Saccharomyces cerevisiae (baker's yeast)
14	N, U (I, P)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	2	UniProt (A0A6A5PX40)	Saccharomyces cerevisiae (baker's yeast)
15	S, V (N, Q)	DNA polymerase epsilon catalytic subunit A	polymer	2222	255992.5	2	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF22912)	Saccharomyces cerevisiae (baker's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
16	AB, CA, EA, FB, IA... (d, 2, 3, f, 5...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	8	Chemie (ATP)
17	CB, DA, EB, HA, HB... (d, 2, e, 4, f...)	ZINC ION	non-polymer		65.4	14	Chemie (ZN)
18	BB, FA, GB, JA, OA... (d, 3, f, 5, 7...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
19	DB, GA, LA, YA (e, 4, 6, c)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

3, b: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

C, J: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Trp -34	-	expression tag
Ser -33	-	expression tag
His -32	-	expression tag
Pro -31	-	expression tag
Gln -30	-	expression tag
Phe -29	-	expression tag
Glu -28	-	expression tag
Lys -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Trp -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Pro -11	-	expression tag
Gln -10	-	expression tag
Phe -9	-	expression tag
Glu -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

E, L: 1 - 167 (UniProt: Q08032)
E, L: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Gly 167	-	linker
Asp 167	-	linker
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	2290708.4
Non-Polymers*	Number of molecules	32
	Total formula weight	6827.8
All*	Total formula weight	2297536.2

*Water molecules are not included.