7Z13

S. cerevisiae CMGE dimer nucleating origin DNA melting

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2, a	DNA replication licensing factor MCM2	polymer	868	98911.5	2	UniProt (A0A6A5Q1S9) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 2
2	3, b	DNA replication licensing factor MCM3	polymer	1006	111720.2	2	UniProt (P24279) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF22207) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 3
3	4, c	DNA replication licensing factor MCM4	polymer	933	105138.4	2	UniProt (P30665) Pfam (PF21128) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB	Saccharomyces cerevisiae (baker's yeast)	Cell division control protein 54
4	5, d	DNA helicase	polymer	775	86505.7	2	UniProt (A0A6A5PUY8) In PDB	Saccharomyces cerevisiae (baker's yeast)
5	6, e	DNA replication licensing factor MCM6	polymer	1017	113110.2	2	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) In PDB	Saccharomyces cerevisiae (baker's yeast)	Minichromosome maintenance protein 6
6	7, f	DNA replication licensing factor MCM7	polymer	845	95049.9	2	UniProt (A0A6A5Q4N0) In PDB	Saccharomyces cerevisiae (baker's yeast)
7	A	DNA (53-MER)	polymer	53	16320.6	1		DNA molecule
8	B	DNA (53-MER)	polymer	53	16311.6	1		DNA molecule
9	C, J	DNA replication complex GINS protein PSF3	polymer	229	25718.1	2	UniProt (Q12146) Pfam (PF22466) Pfam (PF05916) In PDB	Saccharomyces cerevisiae (baker's yeast)	Partner of Sld five 3
10	D, K	DNA replication complex GINS protein SLD5	polymer	294	33983.6	2	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922) In PDB	Saccharomyces cerevisiae (baker's yeast)
11	E, L	Cell division control protein 45	polymer	657	75154.7	2	UniProt (Q08032) Pfam (PF02724) In PDB	Saccharomyces cerevisiae (baker's yeast)
12	F, M	DNA polymerase epsilon subunit B	polymer	689	78425.9	2	UniProt (P24482) Pfam (PF04042) In PDB	Saccharomyces cerevisiae (baker's yeast)	DNA polymerase II subunit 2
13	H, O	DNA replication complex GINS protein PSF1	polymer	208	24230.6	2	UniProt (A0A6A5Q203) In PDB	Saccharomyces cerevisiae (baker's yeast)
14	I, P	DNA replication complex GINS protein PSF2	polymer	213	25096.8	2	UniProt (A0A6A5PX40) In PDB	Saccharomyces cerevisiae (baker's yeast)
15	N, Q	DNA polymerase epsilon catalytic subunit A	polymer	2222	255992.5	2	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF22912) In PDB	Saccharomyces cerevisiae (baker's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
16	d, 2, 3, f, 5...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	8	Chemie (ATP)
17	d, 2, e, 4, f...	ZINC ION	non-polymer		65.4	14	Chemie (ZN)
18	d, 3, f, 5, 7...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
19	e, 4, 6, c	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

3, b: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

C, J: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Trp -34	-	expression tag
Ser -33	-	expression tag
His -32	-	expression tag
Pro -31	-	expression tag
Gln -30	-	expression tag
Phe -29	-	expression tag
Glu -28	-	expression tag
Lys -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Trp -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Pro -11	-	expression tag
Gln -10	-	expression tag
Phe -9	-	expression tag
Glu -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

E, L: 1 - 167 (UniProt: Q08032)
E, L: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Gly 167	-	linker
Asp 167	-	linker
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	2290708.4
Non-Polymers*	Number of molecules	32
	Total formula weight	6827.8
All*	Total formula weight	2297536.2

*Water molecules are not included.