7UXA

Human tRNA Splicing Endonuclease Complex bound to pre-tRNA-ARG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA-splicing endonuclease subunit Sen34	polymer	352	37941.3	1	UniProt (Q9BSV6) Pfam (PF26577) Pfam (PF01974)	Homo sapiens (human)	Leukocyte receptor cluster member 5,tRNA-intron endonuclease Sen34,HsSen34
2	B (B)	tRNA-splicing endonuclease subunit Sen15	polymer	183	19996.6	1	UniProt (Q8WW01) Pfam (PF09631)	Homo sapiens (human)	SEN15 homolog,HsSEN15,tRNA-intron endonuclease Sen15
3	C (C)	tRNA-splicing endonuclease subunit Sen2	polymer	483	55353.0	1	UniProt (Q8NCE0) Pfam (PF02778) Pfam (PF01974)	Homo sapiens (human)	tRNA-intron endonuclease Sen2,HsSen2
4	D (D)	tRNA-splicing endonuclease subunit Sen54	polymer	551	61863.4	1	UniProt (Q7Z6J9) Pfam (PF12928)	Homo sapiens (human)	SEN54 homolog,HsSEN54,tRNA-intron endonuclease Sen54
5	E (E)	RNA (78-MER)	polymer	88	28440.9	1	GenBank (473010)	synthetic construct
6	F, G (E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 309 (UniProt: Q9BSV6)

PDB	External Database	Details
Met -42	-	initiating methionine
Ala -41	-	expression tag
Ser -40	-	expression tag
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Phe -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Ala 247	Tyr 247	engineered mutation
Ala 255	His 255	engineered mutation
Ala 286	Lys 286	engineered mutation

B: 1 - 171 (UniProt: Q8WW01)

PDB	External Database	Details
Gly 172	-	expression tag
Pro 173	-	expression tag
Glu 174	-	expression tag
Gln 175	-	expression tag
Lys 176	-	expression tag
Leu 177	-	expression tag
Ile 178	-	expression tag
Ser 179	-	expression tag
Glu 180	-	expression tag
Glu 181	-	expression tag
Asp 182	-	expression tag
Leu 183	-	expression tag

C: 1 - 465 (UniProt: Q8NCE0)

PDB	External Database	Details
Ala 369	Tyr 369	engineered mutation
Ala 377	His 377	engineered mutation
Ala 416	Lys 416	engineered mutation
Asp 466	-	expression tag
Tyr 467	-	expression tag
Lys 468	-	expression tag
Asp 469	-	expression tag
Asp 470	-	expression tag
Asp 471	-	expression tag
Asp 472	-	expression tag
Lys 473	-	expression tag
Gly 474	-	expression tag
Phe 475	-	expression tag
Trp 476	-	expression tag
Ser 477	-	expression tag
His 478	-	expression tag
Pro 479	-	expression tag
Gln 480	-	expression tag
Phe 481	-	expression tag
Glu 482	-	expression tag
Lys 483	-	expression tag

D: 1 - 526 (UniProt: Q7Z6J9)

PDB	External Database	Details
Gln 527	-	expression tag
Ala 528	-	expression tag
Tyr 529	-	expression tag
Val 530	-	expression tag
Glu 531	-	expression tag
Gln 532	-	expression tag
Lys 533	-	expression tag
Leu 534	-	expression tag
Ile 535	-	expression tag
Ser 536	-	expression tag
Glu 537	-	expression tag
Glu 538	-	expression tag
Asp 539	-	expression tag
Leu 540	-	expression tag
Asn 541	-	expression tag
Ser 542	-	expression tag
Ala 543	-	expression tag
Val 544	-	expression tag
Asp 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	203595.2
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	203643.8

*Water molecules are not included.