7UXA
Human tRNA Splicing Endonuclease Complex bound to pre-tRNA-ARG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | tRNA-splicing endonuclease subunit Sen34 | polymer | 352 | 37941.3 | 1 | UniProt (Q9BSV6) Pfam (PF01974) In PDB | Homo sapiens (human) | Leukocyte receptor cluster member 5,tRNA-intron endonuclease Sen34,HsSen34 |
| 2 | B | tRNA-splicing endonuclease subunit Sen15 | polymer | 183 | 19996.6 | 1 | UniProt (Q8WW01) Pfam (PF09631) In PDB | Homo sapiens (human) | SEN15 homolog,HsSEN15,tRNA-intron endonuclease Sen15 |
| 3 | C | tRNA-splicing endonuclease subunit Sen2 | polymer | 483 | 55353.0 | 1 | UniProt (Q8NCE0) Pfam (PF02778) Pfam (PF01974) In PDB | Homo sapiens (human) | tRNA-intron endonuclease Sen2,HsSen2 |
| 4 | D | tRNA-splicing endonuclease subunit Sen54 | polymer | 551 | 61863.4 | 1 | UniProt (Q7Z6J9) Pfam (PF12928) In PDB | Homo sapiens (human) | SEN54 homolog,HsSEN54,tRNA-intron endonuclease Sen54 |
| 5 | E | RNA (78-MER) | polymer | 88 | 28440.9 | 1 | GenBank (473010) | synthetic construct | |
| 6 | E | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A: 1 - 309 (UniProt: Q9BSV6)
B: 1 - 171 (UniProt: Q8WW01)
C: 1 - 465 (UniProt: Q8NCE0)
D: 1 - 526 (UniProt: Q7Z6J9)
| PDB | External Database | Details |
|---|---|---|
| Met -42 | - | initiating methionine |
| Ala -41 | - | expression tag |
| Ser -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Ala 247 | Tyr 247 | engineered mutation |
| Ala 255 | His 255 | engineered mutation |
| Ala 286 | Lys 286 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 172 | - | expression tag |
| Pro 173 | - | expression tag |
| Glu 174 | - | expression tag |
| Gln 175 | - | expression tag |
| Lys 176 | - | expression tag |
| Leu 177 | - | expression tag |
| Ile 178 | - | expression tag |
| Ser 179 | - | expression tag |
| Glu 180 | - | expression tag |
| Glu 181 | - | expression tag |
| Asp 182 | - | expression tag |
| Leu 183 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 369 | Tyr 369 | engineered mutation |
| Ala 377 | His 377 | engineered mutation |
| Ala 416 | Lys 416 | engineered mutation |
| Asp 466 | - | expression tag |
| Tyr 467 | - | expression tag |
| Lys 468 | - | expression tag |
| Asp 469 | - | expression tag |
| Asp 470 | - | expression tag |
| Asp 471 | - | expression tag |
| Asp 472 | - | expression tag |
| Lys 473 | - | expression tag |
| Gly 474 | - | expression tag |
| Phe 475 | - | expression tag |
| Trp 476 | - | expression tag |
| Ser 477 | - | expression tag |
| His 478 | - | expression tag |
| Pro 479 | - | expression tag |
| Gln 480 | - | expression tag |
| Phe 481 | - | expression tag |
| Glu 482 | - | expression tag |
| Lys 483 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 527 | - | expression tag |
| Ala 528 | - | expression tag |
| Tyr 529 | - | expression tag |
| Val 530 | - | expression tag |
| Glu 531 | - | expression tag |
| Gln 532 | - | expression tag |
| Lys 533 | - | expression tag |
| Leu 534 | - | expression tag |
| Ile 535 | - | expression tag |
| Ser 536 | - | expression tag |
| Glu 537 | - | expression tag |
| Glu 538 | - | expression tag |
| Asp 539 | - | expression tag |
| Leu 540 | - | expression tag |
| Asn 541 | - | expression tag |
| Ser 542 | - | expression tag |
| Ala 543 | - | expression tag |
| Val 544 | - | expression tag |
| Asp 545 | - | expression tag |
| His 546 | - | expression tag |
| His 547 | - | expression tag |
| His 548 | - | expression tag |
| His 549 | - | expression tag |
| His 550 | - | expression tag |
| His 551 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 203595.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 203643.8 |
