7UXA
Human tRNA Splicing Endonuclease Complex bound to pre-tRNA-ARG
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TALOS ARCTICA,FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200,300 |
| Detector | GATAN K2 SUMMIT (4k x 4k),GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.28 |
| Number of Particle | 570104 |
| Software | cryoSPARC,SerialEM,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
