7TVF

Crystal structure of the SHOC2-MRAS-PP1CA (SMP) complex to a resolution of 2.17 Angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (F, C)	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	polymer	329	37426.9	2	UniProt (P62136) Pfam (PF16891) Pfam (PF00149)	Homo sapiens (human)	PP-1A
2	C, D (E, B)	Ras-related protein M-Ras	polymer	179	20426.3	2	UniProt (O14807) Pfam (PF00071)	Homo sapiens (human)	Ras-related protein R-Ras3
3	E, F (A, D)	Leucine-rich repeat protein SHOC-2	polymer	582	64898.7	2	UniProt (Q9UQ13) Pfam (PF13855)	Homo sapiens (human)	Protein soc-2 homolog,Protein sur-8 homolog
4	AA, DA, EA, FA, G... (B, A, F, D, C...)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL)
5	BA, HA, I, J, OA... (B, A, F, D, C...)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
6	K, L, R, S (F, C)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
7	M, Y (F, E)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8	N (F)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
9	IA, JA, KA, O, QA (A, F, D)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
10	T, Z (E, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
11	CA, X (B, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
12	RA, SA, TA, UA, VA... (F, C, E, B, A...)	water	water		18.0	1046	Chemie (HOH)

Sequence modifications

E, B: 1 - 178 (UniProt: O14807)

PDB	External Database	Details
Gly 0	-	expression tag
Leu 71	Gln 71	engineered mutation

A, D: 2 - 582 (UniProt: Q9UQ13)

PDB	External Database	Details
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	245503.8
Non-Polymers*	Number of molecules	37
	Total formula weight	3596.7
All*	Total formula weight	249100.5

*Water molecules are not included.