7TVF
Crystal structure of the SHOC2-MRAS-PP1CA (SMP) complex to a resolution of 2.17 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979110 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 129.938, 129.938, 326.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 163.400 | 2.200 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.137 | 2.007 |
Rpim | 0.077 | 1.228 |
Number of reflections | 148476 | 7129 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.9 | |
Redundancy | 7.9 | |
CC(1/2) | 0.997 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Crystals originally grown in 15% PEG 1500, 0.1M MIB pH 4.2 (15mg/ml of SMP) were used to make seeds. Microseeded into a condition with a reservoir consisting of 17.8% PEG 3350, 136mM sodium sulfate with 1:10 dilution of seeds (7.5mg/ml of SMP) using a ratio of 200nl protein:133nl reservoir:67nl diluted seeds |