7TQL
CryoEM structure of the human 40S small ribosomal subunit in complex with translation initiation factors eIF1A and eIF5B.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | Eukaryotic translation initiation factor 5B | polymer | 619 | 69868.1 | 1 | UniProt (O60841) Pfam (PF00009) Pfam (PF11987) Pfam (PF14578) | Homo sapiens (human) | eIF-5B,Translation initiation factor IF-2 |
| 2 | B (2) | 18S ribosomal RNA | polymer | 1656 | 534544.8 | 1 | Homo sapiens | ||
| 3 | C (3) | Human Met-tRNAiMet | polymer | 75 | 24231.5 | 1 | GenBank (174924) | Homo sapiens (human) | |
| 4 | D (4) | Translation initiation factor eIF1A | polymer | 90 | 10384.0 | 1 | Pfam (PF01176) UniProt (by SIFTS) (P47813) | Homo sapiens | |
| 5 | E, F (B, C) | ribosomal protein uS2 | polymer | 419 | 47843.6 | 2 | Pfam (PF00318) Pfam (PF01015) UniProt (by SIFTS) (P08865) UniProt (by SIFTS) (P61247) | Homo sapiens | |
| 6 | G (D) | 40S ribosomal protein S2 | polymer | 218 | 24091.5 | 1 | UniProt (P15880) Pfam (PF00333) Pfam (PF03719) | Homo sapiens (human) | 40S ribosomal protein S4,Protein LLRep3,Small ribosomal subunit protein uS5 |
| 7 | H (E) | 40S ribosomal protein S4, X isoform | polymer | 258 | 29164.3 | 1 | UniProt (P62701) Pfam (PF08071) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) | Homo sapiens (human) | SCR10,Single copy abundant mRNA protein,Small ribosomal subunit protein eS4 |
| 8 | I (F) | 40S ribosomal protein S3 | polymer | 225 | 24970.3 | 1 | UniProt (P23396) Pfam (PF07650) Pfam (PF00189) | Homo sapiens (human) | Small ribosomal subunit protein uS3 |
| 9 | J (G) | 40S ribosomal protein S6 | polymer | 226 | 26157.9 | 1 | UniProt (P62753) Pfam (PF01092) | Homo sapiens (human) | Phosphoprotein NP33,Small ribosomal subunit protein eS6 |
| 10 | K (H) | 40S ribosomal protein S7 | polymer | 185 | 21187.8 | 1 | UniProt (P62081) Pfam (PF01251) | Homo sapiens (human) | Small ribosomal subunit protein eS7 |
| 11 | L (I) | 40S ribosomal protein S8 | polymer | 205 | 23946.0 | 1 | UniProt (P62241) Pfam (PF01201) | Homo sapiens (human) | Small ribosomal subunit protein eS8 |
| 12 | M (J) | 40S ribosomal protein S9 | polymer | 180 | 21279.3 | 1 | UniProt (P46781) Pfam (PF00163) Pfam (PF01479) | Homo sapiens (human) | Small ribosomal subunit protein uS4 |
| 13 | N (K) | 40S ribosomal protein S5 | polymer | 189 | 21327.7 | 1 | UniProt (P46782) Pfam (PF00177) | Homo sapiens (human) | Small ribosomal subunit protein uS7 |
| 14 | O (L) | 40S ribosomal protein S11 | polymer | 147 | 17170.2 | 1 | UniProt (P62280) Pfam (PF16205) Pfam (PF00366) | Homo sapiens (human) | Small ribosomal subunit protein uS17 |
| 15 | P (M) | 40S ribosomal protein S10 | polymer | 97 | 11616.8 | 1 | UniProt (A0A2R8Y7H1) Pfam (PF03501) UniProt (by SIFTS) (P46783) | Homo sapiens (human) | |
| 16 | Q (N) | ribosomal protein uS15 | polymer | 141 | 16340.3 | 1 | Pfam (PF08069) Pfam (PF00312) UniProt (by SIFTS) (P62277) | Homo sapiens | |
| 17 | R (O) | ribosomal protein eS12 | polymer | 120 | 13347.6 | 1 | Pfam (PF01248) UniProt (by SIFTS) (P25398) | Homo sapiens | |
| 18 | S (P) | ribosomal protein uS11 | polymer | 134 | 14344.4 | 1 | Pfam (PF00411) UniProt (by SIFTS) (P62263) | Homo sapiens | |
| 19 | T (Q) | 40S ribosomal protein S15 | polymer | 115 | 13586.0 | 1 | UniProt (P62841) Pfam (PF00203) | Homo sapiens (human) | RIG protein,Small ribosomal subunit protein uS19 |
| 20 | U (R) | 40S ribosomal protein S16 | polymer | 139 | 15765.5 | 1 | UniProt (P62249) Pfam (PF00380) | Homo sapiens (human) | Small ribosomal subunit protein uS9 |
| 21 | V (S) | ribosomal protein eS17 | polymer | 132 | 15250.7 | 1 | Pfam (PF00833) UniProt (by SIFTS) (P08708) | Homo sapiens | |
| 22 | W (T) | ribosomal protein uS13 | polymer | 140 | 16496.2 | 1 | Pfam (PF00416) UniProt (by SIFTS) (P62269) | Homo sapiens | |
| 23 | X (U) | 40S ribosomal protein S19 | polymer | 139 | 15409.7 | 1 | UniProt (P39019) Pfam (PF01090) | Homo sapiens (human) | Small ribosomal subunit protein eS19 |
| 24 | Y (V) | ribosomal protein uS10 | polymer | 101 | 11508.6 | 1 | Pfam (PF00338) UniProt (by SIFTS) (P60866) | Homo sapiens | |
| 25 | Z (W) | 40S ribosomal protein S15a | polymer | 129 | 14734.4 | 1 | UniProt (P62244) Pfam (PF00410) | Homo sapiens (human) | Small ribosomal subunit protein uS8 |
| 26 | AA (X) | 40S ribosomal protein S23 | polymer | 141 | 15626.4 | 1 | UniProt (P62266) Pfam (PF00164) | Homo sapiens (human) | Small ribosomal subunit protein uS12 |
| 27 | BA (Y) | Isoform 3 of 40S ribosomal protein S24 | polymer | 123 | 14361.0 | 1 | UniProt (P62847) Pfam (PF01282) | Homo sapiens (human) | Small ribosomal subunit protein eS24 |
| 28 | CA (Z) | 40S ribosomal protein S21 | polymer | 82 | 8977.2 | 1 | UniProt (P63220) Pfam (PF01249) | Homo sapiens (human) | Small ribosomal subunit protein eS21 |
| 29 | DA (a) | ribosomal protein eS25 | polymer | 72 | 8182.7 | 1 | Pfam (PF03297) UniProt (by SIFTS) (P62851) | Homo sapiens | |
| 30 | EA (b) | 40S ribosomal protein S27 | polymer | 82 | 9210.8 | 1 | UniProt (P42677) Pfam (PF01667) | Homo sapiens (human) | Metallopan-stimulin 1,MPS-1,Small ribosomal subunit protein eS27 |
| 31 | FA (c) | 40S ribosomal protein S26 | polymer | 99 | 11341.4 | 1 | UniProt (P62854) Pfam (PF01283) | Homo sapiens (human) | Small ribosomal subunit protein eS26 |
| 32 | GA (d) | ribosomal protein eS28 | polymer | 58 | 6451.4 | 1 | Pfam (PF01200) UniProt (by SIFTS) (P62857) | Homo sapiens | |
| 33 | HA (e) | ribosomal protein eS30 | polymer | 51 | 5640.6 | 1 | Pfam (PF04758) UniProt (by SIFTS) (P62861) | Homo sapiens | |
| 34 | IA (f) | ribosomal protein uS14 | polymer | 54 | 6502.6 | 1 | Pfam (PF00253) UniProt (by SIFTS) (P62273) | Homo sapiens | |
| 35 | JA (g) | 40S ribosomal protein S27a | polymer | 65 | 7623.0 | 1 | UniProt (P62979) Pfam (PF01599) | Homo sapiens (human) | Small ribosomal subunit protein eS31 |
| 36 | KA (h) | ribosomal protein eL41 | polymer | 19 | 2683.5 | 1 | Pfam (PF05162) UniProt (by SIFTS) (P62945) | Homo sapiens | |
| 37 | LA (j) | Receptor of activated protein C kinase 1 | polymer | 314 | 34726.2 | 1 | UniProt (P63244) Pfam (PF00400) | Homo sapiens (human) | Cell proliferation-inducing gene 21 protein,Guanine nucleotide-binding protein subunit beta-2-like 1,Guanine nucleotide-binding protein subunit beta-like protein 12.3,Human lung cancer oncogene 7 protein,HLC-7,Receptor for activated C kinase,Small ribosomal subunit protein RACK1 |
| 38 | MA (1) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 39 | NA (3) | GUANOSINE-5'-MONOPHOSPHATE | non-polymer | 363.2 | 1 | Chemie (5GP) | |||
| 40 | OA, PA, QA (c, f, g) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
1: 601 - 1219 (UniProt: O60841)
| PDB | External Database | Details |
|---|---|---|
| Thr 624 | Asn 625 | engineered mutation |
| Glu 625 | - | insertion |
| Lys 626 | Thr 626 | engineered mutation |
| Asp 627 | Glu 627 | engineered mutation |
| Thr 906 | Leu 906 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 38 |
| Total formula weight | 1233737.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1081.6 | |
| All* | Total formula weight | 1234819.2 |
