EMDB-26067: CryoEM structure of the human 40S small ribosomal subunit in comp...

Basic information

Entry

Database: EMDB / ID: EMD-26067

• Title: CryoEM structure of the human 40S small ribosomal subunit in complex with translation initiation factors eIF1A and eIF5B.
• Map data: Sharpened map

Sample

• Cell: human 40S ribosomal subunit in complex with eIF1A and eIF5B
  • RNA: x 2 types
  • Protein or peptide: x 31 types
• Protein or peptide: x 4 types
• Ligand: x 3 types

• Keywords: eIF5B / translation / initiation / eIF1A / RIBOSOME

Function / homology

Function:

negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / positive regulation of ubiquitin-protein transferase activity / positive regulation of respiratory burst involved in inflammatory response / regulation of translational initiation / positive regulation of gastrulation / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / protein tyrosine kinase inhibitor activity / IRE1-RACK1-PP2A complex / protein-synthesizing GTPase / positive regulation of Golgi to plasma membrane protein transport / nucleolus organization / positive regulation of DNA-templated transcription initiation / TNFR1-mediated ceramide production / negative regulation of RNA splicing / neural crest cell differentiation / supercoiled DNA binding / NF-kappaB complex / negative regulation of DNA repair / cytoplasmic translational initiation / oxidized purine DNA binding / cysteine-type endopeptidase activator activity involved in apoptotic process / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / rRNA modification in the nucleus and cytosol / negative regulation of bicellular tight junction assembly / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / negative regulation of phagocytosis / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / protein kinase A binding / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / pigmentation / positive regulation of mitochondrial depolarization / negative regulation of Wnt signaling pathway / fibroblast growth factor binding / monocyte chemotaxis / BH3 domain binding / Protein hydroxylation / negative regulation of translational frameshifting / SARS-CoV-1 modulates host translation machinery / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / TOR signaling / positive regulation of GTPase activity / iron-sulfur cluster binding / mTORC1-mediated signalling / Peptide chain elongation / regulation of cell division / Selenocysteine synthesis / Formation of a pool of free 40S subunits / cellular response to ethanol / Eukaryotic Translation Termination / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / SRP-dependent cotranslational protein targeting to membrane / Response of EIF2AK4 (GCN2) to amino acid deficiency / negative regulation of protein binding / protein serine/threonine kinase inhibitor activity / Viral mRNA Translation / ubiquitin ligase inhibitor activity / negative regulation of respiratory burst involved in inflammatory response / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of signal transduction by p53 class mediator / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of ubiquitin-dependent protein catabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / regulation of translational fidelity / positive regulation of microtubule polymerization / phagocytic cup / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / positive regulation of intrinsic apoptotic signaling pathway / spindle assembly / Protein methylation / Nuclear events stimulated by ALK signaling in cancer / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / positive regulation of cell cycle / translation initiation factor binding / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / translation initiation factor activity / Maturation of protein E / gastrulation / DNA-(apurinic or apyrimidinic site) endonuclease activity / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / IRAK2 mediated activation of TAK1 complex / Prevention of phagosomal-lysosomal fusion / Alpha-protein kinase 1 signaling pathway / signaling adaptor activity / Glycogen synthesis

Domain/homology:

Elongation factor Tu-type domain / Elongation factor Tu domain 4 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S27a / : / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S7e signature. / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S3, eukaryotic/archaeal / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S27e signature. / Ribosomal protein S6, eukaryotic / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S6e signature. / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S8e / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / : / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / : / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Small GTP-binding protein domain / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S7, conserved site / Ubiquitin family

Component:

Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 5B / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS26 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Lapointe CP / Grosely R

Funding support

United States, Sweden, 3 items

Organization	Grant number	Country
Damon Runyon Cancer Research Foundation	DRG-#2321-18	United States
Knut and Alice Wallenberg Foundation	(KAW2015.0406	Sweden
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)	(GM011378	United States

• Citation: Journal: Nature / Year: 2022; Title: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.; Authors: Christopher P Lapointe / Rosslyn Grosely / Masaaki Sokabe / Carlos Alvarado / Jinfan Wang / Elizabeth Montabana / Nancy Villa / Byung-Sik Shin / Thomas E Dever / Christopher S Fraser / Israel S Fernández / Joseph D Puglisi / ; Abstract: Translation initiation defines the identity and quantity of a synthesized protein. The process is dysregulated in many human diseases. A key commitment step is when the ribosomal subunits join at a translation start site on a messenger RNA to form a functional ribosome. Here, we combined single-molecule spectroscopy and structural methods using an in vitro reconstituted system to examine how the human ribosomal subunits join. Single-molecule fluorescence revealed when the universally conserved eukaryotic initiation factors eIF1A and eIF5B associate with and depart from initiation complexes. Guided by single-molecule dynamics, we visualized initiation complexes that contained both eIF1A and eIF5B using single-particle cryo-electron microscopy. The resulting structure revealed how eukaryote-specific contacts between the two proteins remodel the initiation complex to orient the initiator aminoacyl-tRNA in a conformation compatible with ribosomal subunit joining. Collectively, our findings provide a quantitative and architectural framework for the molecular choreography orchestrated by eIF1A and eIF5B during translation initiation in humans.

History

Deposition	Jan 26, 2022	-
Header (metadata) release	Apr 27, 2022	-
Map release	Apr 27, 2022	-
Update	Jun 12, 2024	-
Current status	Jun 12, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_26067.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Sharpened map
• Voxel size: X=Y=Z: 1.31 Å

Density

Contour Level	By AUTHOR: 0.1
Minimum - Maximum	-0.031308204 - 2.5343385
Average (Standard dev.)	0.0004691698 (±0.022956336)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 628.8 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_26067_msk_1.map

Additional map: Unsharpened map

• File: emd_26067_additional_1.map
• Annotation: Unsharpened map

Half map: Half map 1

• File: emd_26067_half_map_1.map
• Annotation: Half map 1

Half map: Half map 2

• File: emd_26067_half_map_2.map
• Annotation: Half map 2

Sample components

Entire : human 40S ribosomal subunit in complex with eIF1A and eIF5B

• Entire: Name: human 40S ribosomal subunit in complex with eIF1A and eIF5B

Components

• Cell: human 40S ribosomal subunit in complex with eIF1A and eIF5B
  • RNA: 18S ribosomal RNA
  • RNA: Human Met-tRNAiMet
  • Protein or peptide: Translation initiation factor eIF1A
  • Protein or peptide: ribosomal protein uS2
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: ribosomal protein uS15
  • Protein or peptide: ribosomal protein eS12
  • Protein or peptide: ribosomal protein uS11
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: ribosomal protein eS17
  • Protein or peptide: ribosomal protein uS13
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: ribosomal protein uS10
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: Isoform 3 of 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: ribosomal protein eS25
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: ribosomal protein eS28
  • Protein or peptide: ribosomal protein eS30
  • Protein or peptide: ribosomal protein eL41
  • Protein or peptide: Receptor of activated protein C kinase 1
• Protein or peptide: Eukaryotic translation initiation factor 5B
• Protein or peptide: 40S ribosomal protein S26
• Protein or peptide: ribosomal protein uS14
• Protein or peptide: 40S ribosomal protein S27a
• Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
• Ligand: GUANOSINE-5'-MONOPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: human 40S ribosomal subunit in complex with eIF1A and eIF5B

• Supramolecule: Name: human 40S ribosomal subunit in complex with eIF1A and eIF5B; type: cell / ID: 1 / Parent: 0 / Macromolecule list: #2-#30, #32-#33, #36-#37
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Eukaryotic translation initiation factor 5B

• Macromolecule: Name: Eukaryotic translation initiation factor 5B / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 69.868062 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

EERAYDKAKR RIEKRRLEHS KNVTEKDKLR APIICVLGHV DTGKTKILDK LRHTHVQDGE AGGITQQIGA TNVPLEAINE QTKMIKNFD RENVRIPGML IIDTPGHESF SNLRNRGSSL CDIAILVVDI MHGLEPQTIE SINLLKSKKC PFIVALNKID R LYDWKKSP DSDVAATLKK QKKNTKDEFE ERAKAIIVEF AQQGLNAALF YENKDPRTFV SLVPTSAHTG DGMGSLIYLL VE LTQTMLS KRLAHCEELR AQVMEVKALP GMGTTIDVIL INGRLKEGDT IIVPGVEGPI VTQIRGLLTP PPMKELRVKN QYE KHKEVE AAQGVKILGK DLEKTLAGLP LLVAYKEDEI PVLKDELIHE LKQTLNAIKL EEKGVYVQAS TLGSLEALLE FLKT SEVPY AGINIGPVHK KDVMKASVML EHDPQYAVIL AFDVRIERDA QEMADSLGVR IFSAEIIYHL FDAFTKYRQD YKKQK QEEF KHIAVFPCKI KILPQYIFNS RDPIVMGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN HKQVDVAKKG QEVCVK IEP IPGESPKMFG RHFEATDILV SKISRQSIDA LKDWFRDEMQ KSDWQLIVEL KKVFEI

UniProtKB: Eukaryotic translation initiation factor 5B

Macromolecule #4: Translation initiation factor eIF1A

• Macromolecule: Name: Translation initiation factor eIF1A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.384011 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

RELVFKEDGQ EYAQVIKMLG NGRLEAMCFD GVKRLCHIRG KLRKKVWINT SDIILVGLRD YQDNKADVIL KYNADEARSL KAYGELPEH A

Macromolecule #5: ribosomal protein uS2

• Macromolecule: Name: ribosomal protein uS2 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.84359 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPVV DPFSKKDWYD VKAPAMFNIR NIGKTLVTRT QG TKIASDG LKGRVFEVSL ADLQNDEVAF RKFKLITEDV QGKNCLTNFH GMDLTRDKMC SMVKKWQTMI EAHVDVKTTD GYL LRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRK VKMLK KPKFELGKLM ELHG

Macromolecule #6: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.091453 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

EWMPVTKLGR LVKDMKIKSL EEIYLFSLPI KESEIIDFFL GASLKDEVLK IMPVQKQTRA GQRTRFKAFV AIGDYNGHVG LGVKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP APRGTGIVSA PVPKKLLMMA G IDDCYTSA RGCTATLGNF AKATFDAISK TYSYLTPDLW KETVFTKSPY QEFTDHLVKT

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #7: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.164336 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNR LKYALTGDEV KKICMQRFIK IDGKVRTDIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI Q IDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RG KGIRLTI AEERDKRLAA K

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #8: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.970289 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIILA TRTQNVLGEK GRRIRELTAV VQKRFGFPEG SVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG VLRFIMESGA KGCEVVVSGK LRGQRAKSMK FVDGLMIHSG D PVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #9: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.157934 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQE

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #10: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.187762 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

IVKPNGEKPD EFESGISQAL LELEMNSDLK AQLRELNITA AKEIEVGGGR KAIIIFVPVP QLKSFQKIQV RLVRELEKKF SGKHVVFIA QRRILPKPTR KSRTKNKQKR PRSRTLTAVH DAILEDLVFP SEIVGKRIRV KLDGSRLIKV HLDKAQQNNV E HKVETFSG VYKKLTGKDV NFEFPEF

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #11: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.945959 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #12: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.279271 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK KG

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #13: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.327723 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

DIKLFGKWST DDVQINDISL QDYIAVKEKY AKYLPHSAGR YAAKRFRKAQ CPIVERLTNS MMMHGRNNGK KLMTVRIVKH AFEIIHLLT GENPLQVLVN AIINSGPRED STRIGRAGTV RRQAVDVSPL RRVNQAIWLL CTGAREAAFR NIKTIAECLA D ELINAAKG SSNSYAIKKK DELERVAKSN R

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #14: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.170248 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

ADIQTERAYQ KQPTIFQNKK RVLLGETGKE KLPRYYKNIG LGFKTPKEAI EGTYIDKKCP FTGNVSIRGR ILSGVVTKMK MQRTIVIRR DYLHYIRKYN RFEKRHKNMS VHLSPCFRDV QIGDIVTVGE CRPLSKTVRF NVLKVTKA

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #15: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.61676 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRS

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #16: ribosomal protein uS15

• Macromolecule: Name: ribosomal protein uS15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.340327 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YE

Macromolecule #17: ribosomal protein eS12

• Macromolecule: Name: ribosomal protein eS12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.347567 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GVMDVNTALQ EVLKTALIHD GLARGIREAA KALDKRQAHL CVLASNCDEP MYVKLVEALC AEHQINLIKV DDNKKLGEWV GLCKIDREG KPRKVVGCSC VVVKDYGKES QAKDVIEEYF K

Macromolecule #18: ribosomal protein uS11

• Macromolecule: Name: ribosomal protein uS11 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.344424 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GPQVAEGENV FGVCHIFASF NDTFVHVTDL SGKETICRVT GGMKVKADRD ESSPYAAMLA AQDVAQRCKE LGITALHIKL RATGGNRTK TPGPGAQSAL RALARSGMKI GRIEDVTPIP SDSTRRKGGR RGRRL

Macromolecule #19: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.586022 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

FTYRGVDLDQ LLDMSYEQLM QLYSARQRRR LNRGLRRKQH SLLKRLRKAK KEAPPMEKPE VVKTHLRDMI ILPEMVGSMV GVYNGKTFN QVEIKPEMIG HYLGEFSITY KPVKHG

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #20: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.76548 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

QSVQVFGRKK TATAVAHCKR GNGLIKVNGR PLEMIEPRTL QYKLLEPVLL LGKERFAGVD IRVRVKGGGH VAQIYAIRQS ISKALVAYY QKYVDEASKK EIKDILIQYD RTLLVADPRR CESKKFGGPG ARARYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #21: ribosomal protein eS17

• Macromolecule: Name: ribosomal protein eS17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.250714 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGMNFKT PRG

Macromolecule #22: ribosomal protein uS13

• Macromolecule: Name: ribosomal protein uS13 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.496223 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

LVIPEKFQHI LRVLNTNIDG RRKIAFAITA IKGVGRRYAH VVLRKADIDL TKRAGELTED EVERVITIMQ NPRQYKIPDW FLNRQKDVK DGKYSQVLAN GLDNKLREDL ERLKKIRAHR GLRHFWGLRV RGQHTKTTGR R

Macromolecule #23: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.409689 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

VTVKDVNQQE FVRALAAFLK KSGKLKVPEW VDTVKLAKHK ELAPYDENWF YTRAASTARH LYLRGGAGVG SMTKIYGGRQ RNGVMPSHF SRGSKSVARR VLQALEGLKM VEKDQDGGRK LTPQGQRDLD RIAGQVAAAN

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #24: ribosomal protein uS10

• Macromolecule: Name: ribosomal protein uS10 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.508648 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

IHRIRITLTS RNVKSLEKVC ADLIRGAKEK NLKVKGPVRM PTKTLRITTR KTPCGEGSKT WDRFQMRIHK RLIDLHSPSE IVKQITSIS IEPGVEVEVT IA

Macromolecule #25: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.734357 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #26: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.626392 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #27: Isoform 3 of 40S ribosomal protein S24

• Macromolecule: Name: Isoform 3 of 40S ribosomal protein S24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.361046 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

TVTIRTRKFM TNRLLQRKQM VIDVLHPGKA TVPKTEIREK LAKMYKTTPD VIFVFGFRTH FGGGKTTGFG MIYDSLDYAK KNEPKHRLA RHGLYEKKKT SRKQRKERKN RMKKVRGTAK ANVG

UniProtKB: Small ribosomal subunit protein eS24

Macromolecule #28: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.977214 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KN

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #29: ribosomal protein eS25

• Macromolecule: Name: ribosomal protein eS25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.182693 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQEL LSKGLIKLVS KHRAQVIYTR NT

Macromolecule #30: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.210843 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

PLAKDLLHPS PEEEKRKHKK KRLVQSPNSY FMDVKCPGCY KITTVFSHAQ TVVLCVGCST VLCQPTGGKA RLTEGCSFRR KQ

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #31: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.341426 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #32: ribosomal protein eS28

• Macromolecule: Name: ribosomal protein eS28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.451396 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

PIKLARVTKV LGRTGSQGQC TQVRVEFMDD TSRSIIRNVK GPVREGDVLT LLESEREA

Macromolecule #33: ribosomal protein eS30

• Macromolecule: Name: ribosomal protein eS30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 5.640648 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

AAAAAARAGK VRGQTPKVAK QEKKKKKTGR AKRRMQYNRR FVNVKGPNAN S

Macromolecule #34: ribosomal protein uS14

• Macromolecule: Name: ribosomal protein uS14 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.502573 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

HQQLYWSHPR KFGQGSRSCR VCSNRHGLIR KYGLNMCRQC FRQYAKDIGF IKLD

Macromolecule #35: 40S ribosomal protein S27a

• Macromolecule: Name: 40S ribosomal protein S27a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.623009 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

YTTPKKNKHK RKKVKLAVLK YYKVDENGKI SRLRRECPSD ECGAGVFMAS HFDRHYCGKC CLTYC

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #36: ribosomal protein eL41

• Macromolecule: Name: ribosomal protein eL41 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 2.683481 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MRAKWRKKRM RRLKRKRRK

Macromolecule #37: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.726164 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTIG

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #2: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 534.54475 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GUACUUGGAU AACUGUGGUA AUUCUAGAGC U AAUACAUG CCGACGGGCG CUGACCCCCU UCGCGGGGGG GAUGCGUGCA UUUAUCAGUG GUGACUCUAG AUAACCUCGG GC CGAUCGC ACGCCGGCGG CGACGACCCA UUCGAACGUC UGCCCUAUCA ACUUUCGAUG GUAGUCGCCG UGCCUACCAU GGU GACCAC GGGUGACGGG GAAUCAGGGU UCGAUUCCGG AGAGGGAGCC UGAGAAACGG CUACCACAUC CAAGGAAGGC AGCA GGCGC GCAAAUUACC CACUCCCGAC CCGGGGAGGU AGUGACGAAA AAUAACAAUA CAGGACUCUU UCGAGGCCCU GUAAU UGGA AUGAGUCCAC UUUAAAUCCU UUAACGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGC UCC AAUAGCGUAU AUUAAAGUUG CUGCAGUUAA AAAGCUCGUA GUUGGAUCUC UAGCGUUUAC UUUGAAAAAA UUAGAGU GU UCAAAGCAGG CCGCCUGGAU ACCGCAGCUA GGAAUAAUGG AAUAGGACCG CGGUUCUAUU UUGUUGGUUU UCGGAACU G AGGCCAUGAU UAAGAGGGAC GGCCGGGGGC AUUCGUAUUG CGCCGCUAGA GGUGAAAUUC UUGGACCGGC GCAAGACGG ACCAGAGCGA AAGCAUUUGC CAAGAAUGUU UUCAUUAAUC AAGAACGAAA GUCGGAGGUU CGAAGACGAU CAGAUACCGU CGUAGUUCC GACCAUAAAC GAUGCCGACC GGCGAUGCGG CGGCGUUAUU CCCAUGACCC GCCGGGCAGC UUCCGGGAAA C CAAAGUCU UUGGGUUCCG GGGGGAGUAU GGUUGCAAAG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UG GAGCCUG CGGCUUAAUU UGACUCAACA CGGGAAACCU CACCCGGCCC GGACACGGAC AGGAUUGACA GAUUGAUAGC UCU UUCUCG AUUCCGUGGG UGGUGGUGCA UGGCCGUUCU UAGUUGGUGG AGCGAUUUGU CUGGUUAAUU CCGAUAACGA ACGA GACUC UGGCAUGCUA ACUAGUUACG CGACCGGUCG GCGUAACUUC UUAGAGGGAC AAGUGGCGUU CAGCCACCCG AGAUU GAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUC CUACGC CGG CAGGCGCGGG UAACCCGUUG AACCCCAUUC GUGAUGGGGA UCGGGGAUUG CAAUUAUUCC CCAUGAACGA GGAAUUC CC AGUAAGUGCG GGUCAUAAGC UUGCGUUGAU UAAGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUACUAC CGAUUGGA U GGUUUAGUGA GGCCCUCGGA UCGGCCCCGC CGGGGUGCCC UGGCGGAGCG CUGAGAAGAC GGUCGAACUU GACUAUCUA GAGGAAGUAA AAGUCGUAAC AAGGUUUCCG UAGGUGAACC UGCGGAAGGA UCAUUA

Macromolecule #3: Human Met-tRNAiMet

• Macromolecule: Name: Human Met-tRNAiMet / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.23151 KDa

Sequence

String:

AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

GENBANK: GENBANK: K00328.1

Macromolecule #38: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

• Macromolecule: Name: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 38 / Number of copies: 1 / Formula: GNP
• Molecular weight: Theoretical: 522.196 Da

Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Macromolecule #39: GUANOSINE-5'-MONOPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: 5GP
• Molecular weight: Theoretical: 363.221 Da

Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

Macromolecule #40: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 40 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.2
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 190000
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: PROJECTION MATCHING