7T4S
CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with NRP2 and neutralizing fabs 8I21 and 13H11
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Envelope glycoprotein H | polymer | 767 | 87311.3 | 1 | UniProt (F5H9T3) Pfam (PF02489) Pfam (PF17488) UniProt (by SIFTS) (Q6SW67) | Human betaherpesvirus 5 | gH |
| 2 | B (B) | Envelope glycoprotein L | polymer | 278 | 30846.5 | 1 | UniProt (Q71DN9) Pfam (PF01801) UniProt (by SIFTS) (F5HCH8) | Human betaherpesvirus 5 | gL |
| 3 | C (C) | Envelope protein UL128 | polymer | 171 | 19746.0 | 1 | UniProt (Q38LY2) UniProt (by SIFTS) (V9LLX6) | Human betaherpesvirus 5 | UL128 |
| 4 | D (D) | Envelope glycoprotein UL130 | polymer | 254 | 28866.8 | 1 | UniProt (Q38M07) Pfam (PF11668) UniProt (by SIFTS) (F5HCP3) | Human betaherpesvirus 5 | UL130 |
| 5 | E (E) | Envelope protein UL131A | polymer | 129 | 15011.0 | 1 | UniProt (Q8AZ45) UniProt (by SIFTS) (F5HET4) | Human betaherpesvirus 5 | Protein UL131A,UL131A |
| 6 | F (F) | Neuropilin-2 | polymer | 875 | 98923.2 | 1 | UniProt (O60462) Pfam (PF00431) Pfam (PF00754) Pfam (PF00629) | Homo sapiens (human) | Vascular endothelial cell growth factor 165 receptor 2 |
| 7 | G (H) | Fab 8I21 heavy chain | polymer | 249 | 26816.3 | 1 | Homo sapiens | ||
| 8 | H (J) | Fab 13H11 heavy chain | polymer | 250 | 26600.1 | 1 | Homo sapiens | ||
| 9 | I (K) | Fab 13H11 light chain | polymer | 237 | 25780.0 | 1 | Homo sapiens | ||
| 10 | J (I) | Fab 8I21 light chain | polymer | 238 | 25995.1 | 1 | Homo sapiens | ||
| 11 | K, L, M, N, O... (A, D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) | |||
| 12 | T (F) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 715 (UniProt: F5H9T3)
D: 1 - 214 (UniProt: Q38M07)
F: 1 - 864 (UniProt: O60462)
| PDB | External Database | Details |
|---|---|---|
| Gly 716 | - | expression tag |
| Thr 717 | - | expression tag |
| Lys 718 | - | expression tag |
| Leu 719 | - | expression tag |
| Gly 720 | - | expression tag |
| Pro 721 | - | expression tag |
| Glu 722 | - | expression tag |
| Gln 723 | - | expression tag |
| Lys 724 | - | expression tag |
| Leu 725 | - | expression tag |
| Ile 726 | - | expression tag |
| Ser 727 | - | expression tag |
| Glu 728 | - | expression tag |
| Glu 729 | - | expression tag |
| Asp 730 | - | expression tag |
| Leu 731 | - | expression tag |
| Asn 732 | - | expression tag |
| Ser 733 | - | expression tag |
| Ala 734 | - | expression tag |
| Val 735 | - | expression tag |
| Asp 736 | - | expression tag |
| Gly 737 | - | expression tag |
| Ser 738 | - | expression tag |
| Gly 739 | - | expression tag |
| Leu 740 | - | expression tag |
| Asn 741 | - | expression tag |
| Asp 742 | - | expression tag |
| Ile 743 | - | expression tag |
| Phe 744 | - | expression tag |
| Glu 745 | - | expression tag |
| Ala 746 | - | expression tag |
| Gln 747 | - | expression tag |
| Lys 748 | - | expression tag |
| Ile 749 | - | expression tag |
| Glu 750 | - | expression tag |
| Trp 751 | - | expression tag |
| His 752 | - | expression tag |
| Glu 753 | - | expression tag |
| Asn 754 | - | expression tag |
| Leu 755 | - | expression tag |
| Tyr 756 | - | expression tag |
| Phe 757 | - | expression tag |
| Gln 758 | - | expression tag |
| Gly 759 | - | expression tag |
| His 760 | - | expression tag |
| His 761 | - | expression tag |
| His 762 | - | expression tag |
| His 763 | - | expression tag |
| His 764 | - | expression tag |
| His 765 | - | expression tag |
| His 766 | - | expression tag |
| His 767 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 215 | - | expression tag |
| Ser 216 | - | expression tag |
| Glu 217 | - | expression tag |
| Asn 218 | - | expression tag |
| Leu 219 | - | expression tag |
| Tyr 220 | - | expression tag |
| Phe 221 | - | expression tag |
| Gln 222 | - | expression tag |
| Gly 223 | - | expression tag |
| Ser 224 | - | expression tag |
| Ala 225 | - | expression tag |
| Trp 226 | - | expression tag |
| Ser 227 | - | expression tag |
| His 228 | - | expression tag |
| Pro 229 | - | expression tag |
| Gln 230 | - | expression tag |
| Phe 231 | - | expression tag |
| Glu 232 | - | expression tag |
| Lys 233 | - | expression tag |
| Gly 234 | - | expression tag |
| Gly 235 | - | expression tag |
| Gly 236 | - | expression tag |
| Ser 237 | - | expression tag |
| Gly 238 | - | expression tag |
| Gly 239 | - | expression tag |
| Gly 240 | - | expression tag |
| Ser 241 | - | expression tag |
| Gly 242 | - | expression tag |
| Gly 243 | - | expression tag |
| Gly 244 | - | expression tag |
| Ser 245 | - | expression tag |
| Ala 246 | - | expression tag |
| Trp 247 | - | expression tag |
| Ser 248 | - | expression tag |
| His 249 | - | expression tag |
| Pro 250 | - | expression tag |
| Gln 251 | - | expression tag |
| Phe 252 | - | expression tag |
| Glu 253 | - | expression tag |
| Lys 254 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 865 | - | expression tag |
| Asn 866 | - | expression tag |
| Ser 867 | - | expression tag |
| Asp 868 | - | expression tag |
| Tyr 869 | - | expression tag |
| Lys 870 | - | expression tag |
| Asp 871 | - | expression tag |
| Asp 872 | - | expression tag |
| Asp 873 | - | expression tag |
| Asp 874 | - | expression tag |
| Lys 875 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 385896.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2031.0 | |
| All* | Total formula weight | 387927.3 |
