7T0W

Complex of GABA-A synaptic receptor with autoimmune antibody Fab115

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Gamma-aminobutyric acid receptor subunit beta-2	polymer	340	39407.6	2	UniProt (P47870) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-2
2	B, D (B, D)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	347	39831.8	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
3	E (E)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	394	45354.7	1	UniProt (P18507) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2
4	F, I (L2, L)	Fab115 light chain, IgG1	polymer	217	22838.2	2		Homo sapiens (Human)
5	G, H (H, H2)	Fab115 Heavy Chain, IgG1	polymer	246	26625.5	2		Homo sapiens (Human)
6	J, K, M, N (F, G, J, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
7	L, O (I, M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
8	P (N)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	1	In PDB GlyTouCan (G40702WU)

Sequence modifications

A, C: 1 - 307 (UniProt: P47870)
A, C: 317 - 340 (UniProt: P47870)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker
Ala 315	-	linker
Ile 316	-	linker

B, D: 1 - 312 (UniProt: P14867)
B, D: 320 - 347 (UniProt: P14867)

PDB	External Database	Details
Ser 313	-	linker
Gln 314	-	linker
Pro 315	-	linker
Ala 316	-	linker
Arg 317	-	linker
Ala 318	-	linker
Ala 319	-	linker

E: 1 - 322 (UniProt: P18507)

PDB	External Database	Details
Trp -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
Pro -33	-	expression tag
Gln -32	-	expression tag
Phe -31	-	expression tag
Glu -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

E: 330 - 357 (UniProt: P18507)

PDB	External Database	Details
Ser 323	-	linker
Gln 324	-	linker
Pro 325	-	linker
Ala 326	-	linker
Arg 327	-	linker
Ala 328	-	linker
Ala 329	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	302761.0
Branched	Number of molecules	7
	Total formula weight	4592.2
All*	Total formula weight	307353.2

*Water molecules are not included.