7SOL

Crystal Structures of the bispecific ubiquitin/FAT10 activating enzyme, Uba6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-like modifier-activating enzyme 6	polymer	1020	114760.2	2	UniProt (A0AVT1) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358)	Homo sapiens (Human)	Ubiquitin-activating enzyme 6,Monocyte protein 4,MOP-4,Ubiquitin-activating enzyme E1-like protein 2,E1-L2
2	B, D (B, D)	Ubiquitin	polymer	83	9692.9	2	UniProt (P69326) Pfam (PF00240)	Triticum aestivum (Wheat)
3	E (A)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
4	F, G (B, D)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
5	H, I, J, K (A, B, C, D)	water	water		18.0	847	Chemie (HOH)

Sequence modifications

A, C: 37 - 1052 (UniProt: A0AVT1)

PDB	External Database	Details
Gly 33	-	expression tag
Ala 34	-	expression tag
Met 35	-	expression tag
Gly 36	-	expression tag
Ala 625	Cys 625	engineered mutation

B, D: 1 - 76 (UniProt: P69326)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Arg 6	Lys 6	engineered mutation
Arg 11	Lys 11	engineered mutation
Arg 27	Lys 27	engineered mutation
Arg 29	Lys 29	engineered mutation
Arg 33	Lys 33	engineered mutation
Arg 48	Lys 48	engineered mutation
Arg 63	Lys 63	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	248906.2
Non-Polymers*	Number of molecules	3
	Total formula weight	1354.5
All*	Total formula weight	250260.7

*Water molecules are not included.