7SOL
Crystal Structures of the bispecific ubiquitin/FAT10 activating enzyme, Uba6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2019-10-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 248.572, 101.317, 122.921 |
Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
Resolution | 45.905 - 2.250 |
R-factor | 0.166435680207 |
Rwork | 0.166 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dc6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.718 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 125930 | 5797 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 98.0 | |
Redundancy | 4.75 | |
CC(1/2) | 0.996 | 0.777 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 % PEG 3350, 0.2 M NaF |