7O56

X-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to an interferon-stimulated response element solved by Phosphorus and Sulphur SAD methods

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, E (A, B, C)	Interferon regulatory factor 4	polymer	141	16306.5	3	UniProt (Q15306) Pfam (PF00605)	Homo sapiens (Human)	IRF-4,Lymphocyte-specific interferon regulatory factor,LSIRF,Multiple myeloma oncogene 1,NF-EM5
2	B (D)	DNA (5'-D(P*AP*AP*TP*AP*AP*AP*AP*GP*AP*AP*AP*CP*CP*GP*AP*AP*AP*GP*TP*AP*A)-3')	polymer	21	6514.3	1		synthetic construct
3	C (E)	DNA (5'-D(P*TP*TP*TP*AP*CP*TP*TP*TP*CP*GP*GP*TP*TP*TP*CP*TP*TP*TP*TP*AP*T)-3')	polymer	21	6366.1	1		synthetic construct

Sequence modifications

A, B, C: 20 - 139 (UniProt: Q15306)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ala 9	-	expression tag
Ala 10	-	expression tag
Leu 11	-	expression tag
Glu 12	-	expression tag
Val 13	-	expression tag
Leu 14	-	expression tag
Phe 15	-	expression tag
Gln 16	-	expression tag
Gly 17	-	expression tag
Pro 18	-	expression tag
Gly 19	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	61799.8
All*	Total formula weight	61799.8

*Water molecules are not included.