7M0E

Pre-catalytic synaptic complex of DNA Polymerase Lambda with gapped DSB substrate and incoming dUMPNPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, B, C, D)	DNA polymerase lambda	polymer	348	38719.2	4	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (Human)	Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa
2	B, F, J, N (G, K, O, T)	DNA (5'-D(*CP*GP*GP*CP*AP*GP*C)-3')	polymer	7	2123.4	4		synthetic construct
3	C, G, K, O (F, J, N, R)	DNA (5'-D(*CP*AP*GP*TP*GP*C)-3')	polymer	6	1809.2	4		synthetic construct
4	D, H, L, P (H, L, P, U)	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	4		synthetic construct
5	BA, HA, Q, Y (C, D, A, B)	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	4	Chemie (DUP)
6	CA, FA, R, U, W... (N, D, A, F, B...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
7	AA, GA, S, X (C, D, A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
8	EA, T (N, A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
9	DA, V (N, F)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
10	IA, JA, KA, LA, MA... (A, G, F, H, B...)	water	water		18.0	354	Chemie (HOH)

Sequence modifications

A, B, C, D: 232 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Gly 228	-	expression tag
Ser 229	-	expression tag
Ala 230	-	expression tag
Ala 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	175374.4
Non-Polymers*	Number of molecules	18
	Total formula weight	2422.7
All*	Total formula weight	177797.1

*Water molecules are not included.