7M0E

Pre-catalytic synaptic complex of DNA Polymerase Lambda with gapped DSB substrate and incoming dUMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	64.966, 85.819, 91.421
Unit cell angles	105.22, 92.58, 111.30

Refinement procedure

Resolution	38.160 - 2.250
R-factor	0.1854
Rwork	0.183
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pfo
RMSD bond length	0.005
RMSD bond angle	0.681
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	75755	3835
<I/σ(I)>	17.38	1.73
Completeness [%]	91.9	93.6
Redundancy	3.8	3.7
CC(1/2)	0.986	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.138M sodium sulfate, 20% (w/v) PEG 3350