7KX0

Crystal structure of the CD27:CD70 co-stimulatory complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	CD70 antigen	polymer	158	17482.7	3	UniProt (P32970) Pfam (PF00229) In PDB	Homo sapiens (Human)	CD27 ligand,CD27-L,Tumor necrosis factor ligand superfamily member 7
2	D, E, F	CD27 antigen	polymer	114	12782.4	3	UniProt (P26842) Pfam (PF00020) In PDB	Homo sapiens (Human)	CD27L receptor,T-cell activation antigen CD27,T14,Tumor necrosis factor receptor superfamily member 7
3	G, I	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	2	In PDB GlyTouCan (G32152BH)
4	H	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
5	J, L	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
6	K	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G00395TQ)
7	C	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
8	D, E, F	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
9		water	water		18.0	145	Chemie (HOH)

Sequence modifications

A, B, C: 45 - 193 (UniProt: P32970)

PDB	External Database	Details
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
Gly 42	-	expression tag
Ser 43	-	expression tag
Gly 44	-	expression tag

D, E, F: 23 - 127 (UniProt: P26842)

PDB	External Database	Details
Gly 128	-	expression tag
Ser 129	-	expression tag
Gly 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	90795.5
Branched	Number of molecules	6
	Total formula weight	4930.5
Non-Polymers*	Number of molecules	4
	Total formula weight	785.8
All*	Total formula weight	96511.8

*Water molecules are not included.