7E1X

Cryo-EM structure of hybrid respiratory supercomplex consisting of Mycobacterium tuberculosis complexIII and Mycobacterium smegmatis complexIV in presence of TB47

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, H (E, Q)	Cytochrome c oxidase subunit 2	polymer	341	38077.5	2	UniProt (A0R057) Pfam (PF00116)	Mycobacterium smegmatis MC2 51	Cytochrome C oxidase subunit II ctaC
2	B, I (F, R)	Cytochrome c oxidase subunit 1	polymer	575	64163.0	2		Mycolicibacterium smegmatis MC2 51
3	C, J (G, S)	Cytochrome c oxidase subunit 3	polymer	203	22196.9	2	UniProt (A0R049) Pfam (PF00510)	Mycobacterium smegmatis MC2 51	Cytochrome c oxidase subunit 3 CtaE
4	D, K (H, T)	Cytochrome c oxidase polypeptide 4	polymer	139	15177.4	2	UniProt (A0R056) Pfam (PF12270)	Mycobacterium smegmatis MC2 51	Cytochrome aa3 subunit 4,Cytochrome c oxidase polypeptide IV
5	E, L (I, U)	Cytochrome c oxidase subunit CtaJ	polymer	79	8365.5	2	UniProt (A0R1B6)	Mycobacterium smegmatis MC2 51
6	F, M (J, V)	Uncharacterized protein MSMEG_4692/MSMEI_4575	polymer	157	15911.0	2	UniProt (A0R1B5) Pfam (PF17174)	Mycobacterium smegmatis MC2 51	Cytochrome c oxidase subunit CtaI
7	G, N (D, P)	Prokaryotic respiratory supercomplex associate factor 1 PRSAF1	polymer	100	11329.9	2		Mycolicibacterium smegmatis MC2 51
8	O, R (N, B)	Cytochrome bc1 complex cytochrome b subunit	polymer	549	61077.1	2	UniProt (P9WP37) Pfam (PF13631)	Mycobacterium tuberculosis H37Rv	Cytochrome bc1 reductase complex subunit QcrB,Ubiquinol--cytochrome c reductase cytochrome b subunit
9	P, S (M, A)	Cytochrome bc1 complex Rieske iron-sulfur subunit	polymer	429	46976.5	2	UniProt (P9WH23) Pfam (PF19297) Pfam (PF00355)	Mycobacterium tuberculosis H37Rv	Cytochrome bc1 reductase complex subunit QcrA,Rieske iron-sulfur protein,Ubiquinol--cytochrome c reductase iron-sulfur subunit
10	Q, T (O, C)	Cytochrome bc1 complex cytochrome c subunit	polymer	280	29152.4	2	UniProt (P9WP35) Pfam (PF00034) Pfam (PF13442)	Mycobacterium tuberculosis H37Rv	Cytochrome bc1 reductase complex subunit QcrC,Ubiquinol--cytochrome c reductase cytochrome c subunit
11	AA, FA, GA, KA, LA... (F, Q, R, E)	COPPER (II) ION	non-polymer		63.5	8	Chemie (CU)
12	AB, DB, EA, HA, IA... (M, O, D, R, B...)	CARDIOLIPIN	non-polymer		1464.0	17	Chemie (CDL)
13	JA, LB, SA, Y (R, B, N, F)	PALMITIC ACID	non-polymer		256.4	4	Chemie (PLM)
14	BA, CA, MA, NA (F, R)	HEME-A	non-polymer		852.8	4	Chemie (HEA)
15	DA, OA (G, S)	(2R)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (E)-octadec-9-enoate	non-polymer		718.0	2	Chemie (9Y0)
16	IB, JB, QA, RA (B, N)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
17	AC, EB, FB, QB, RB... (C, O, B, A, N)	MENAQUINONE-9	non-polymer		785.2	8	Chemie (MQ9)
18	SB, YA (B, N)	5-methoxy-2-methyl-~{N}-[[4-[4-[4-(trifluoromethyloxy)phenyl]piperidin-1-yl]phenyl]methyl]pyrazolo[1,5-a]pyridine-3-carboxamide	non-polymer		538.6	2	Chemie (HV0)
19	TB, ZA (A, M)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
20	BB, CB, VB, XB (M, A)	(2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate	non-polymer		853.1	4	Chemie (9YF)
21	GB, HB, YB, ZB (O, C)	HEME C	non-polymer		618.5	4	Chemie (HEC)

Sequence modifications

O, C: 2 - 280 (UniProt: P9WP35)

PDB	External Database	Details
Leu 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	624854.1
Non-Polymers*	Number of molecules	59
	Total formula weight	47333.2
All*	Total formula weight	672187.3

*Water molecules are not included.