7B5L
Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: NEDD8-CUL1-RBX1-SKP1-SKP2-CKSHS1-Cyclin A-CDK2-p27-UBE2L3~Ub~ARIH1. Transition State 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | C | Cullin-1 | polymer | 776 | 89800.4 | 1 | UniProt (Q13616) Pfam (PF00888) Pfam (PF10557) In PDB | Homo sapiens (Human) | CUL-1 |
| 2 | H | E3 ubiquitin-protein ligase ARIH1 | polymer | 557 | 64197.8 | 1 | UniProt (Q9Y4X5) Pfam (PF21235) Pfam (PF00097) Pfam (PF01485) Pfam (PF22191) Pfam (PF19422) In PDB | Homo sapiens (Human) | H7-AP2,HHARI,Monocyte protein 6,MOP-6,Protein ariadne-1 homolog,ARI-1,UbcH7-binding protein,UbcM4-interacting protein,Ubiquitin-conjugating enzyme E2-binding protein 1 |
| 3 | T | S-phase kinase-associated protein 2 | polymer | 424 | 47817.8 | 1 | UniProt (Q13309) Pfam (PF12937) In PDB | Homo sapiens (Human) | Cyclin-A/CDK2-associated protein p45,F-box protein Skp2,F-box/LRR-repeat protein 1,p45skp2 |
| 4 | K | Cyclin-dependent kinases regulatory subunit 1 | polymer | 73 | 8968.3 | 1 | UniProt (P61024) Pfam (PF01111) In PDB | Homo sapiens (Human) | CKS-1 |
| 5 | S | S-phase kinase-associated protein 1 | polymer | 163 | 18680.0 | 1 | UniProt (P63208) Pfam (PF03931) Pfam (PF01466) In PDB | Homo sapiens (Human) | Cyclin-A/CDK2-associated protein p19,p19A,Organ of Corti protein 2,OCP-2,Organ of Corti protein II,OCP-II,RNA polymerase II elongation factor-like protein,SIII,Transcription elongation factor B polypeptide 1-like,p19skp1 |
| 6 | U | Polyubiquitin-C | polymer | 75 | 8519.8 | 1 | UniProt (P0CG48) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
| 7 | N | NEDD8 | polymer | 76 | 8574.0 | 1 | UniProt (Q15843) Pfam (PF00240) In PDB | Homo sapiens (Human) | Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8 |
| 8 | R | E3 ubiquitin-protein ligase RBX1 | polymer | 108 | 12290.0 | 1 | UniProt (P62877) Pfam (PF12678) In PDB | Homo sapiens (Human) | E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1 |
| 9 | D | Ubiquitin-conjugating enzyme E2 L3 | polymer | 154 | 17825.5 | 1 | UniProt (P68036) Pfam (PF00179) In PDB | Homo sapiens (Human) | E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3 |
| 10 | L | Cyclin-dependent kinase 2 | polymer | 298 | 34056.5 | 1 | UniProt (P24941) Pfam (PF00069) In PDB | Homo sapiens (Human) | Cell division protein kinase 2,p33 protein kinase |
| 11 | Y | Cyclin-A2 | polymer | 432 | 48609.6 | 1 | UniProt (P20248) Pfam (PF16500) Pfam (PF00134) Pfam (PF02984) In PDB | Homo sapiens (Human) | Cyclin-A,Cyclin A |
| 12 | P | Cyclin-dependent kinase inhibitor 1B | polymer | 198 | 22188.3 | 1 | UniProt (P46527) Pfam (PF02234) In PDB | Homo sapiens (Human) | Cyclin-dependent kinase inhibitor p27,p27Kip1 |
| 13 | H, R | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 14 | U | 5-azanylpentan-2-one | non-polymer | 101.1 | 1 | Chemie (SY8) |
Sequence modifications
D: 1 - 154 (UniProt: P68036)
| PDB | External Database | Details |
|---|---|---|
| Ala 17 | Cys 17 | engineered mutation |
| Ala 137 | Cys 137 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 381527.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 689.8 | |
| All* | Total formula weight | 382217.5 |
