7B5L

Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: NEDD8-CUL1-RBX1-SKP1-SKP2-CKSHS1-Cyclin A-CDK2-p27-UBE2L3~Ub~ARIH1. Transition State 1

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006513	biological_process	protein monoubiquitination
C	0006915	biological_process	apoptotic process
C	0008283	biological_process	cell population proliferation
C	0009887	biological_process	animal organ morphogenesis
C	0016567	biological_process	protein ubiquitination
C	0019005	cellular_component	SCF ubiquitin ligase complex
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0070936	biological_process	protein K48-linked ubiquitination
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	1990452	cellular_component	Parkin-FBXW7-Cul1 ubiquitin ligase complex
D	0000151	cellular_component	ubiquitin ligase complex
D	0000209	biological_process	protein polyubiquitination
D	0003713	molecular_function	transcription coactivator activity
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006355	biological_process	regulation of DNA-templated transcription
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0008283	biological_process	cell population proliferation
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0019787	molecular_function	ubiquitin-like protein transferase activity
D	0019899	molecular_function	enzyme binding
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032446	biological_process	protein modification by small protein conjugation
D	0036211	biological_process	protein modification process
D	0044770	biological_process	cell cycle phase transition
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
D	0061631	molecular_function	ubiquitin conjugating enzyme activity
D	0070979	biological_process	protein K11-linked ubiquitination
D	0071383	biological_process	cellular response to steroid hormone stimulus
D	0071385	biological_process	cellular response to glucocorticoid stimulus
D	0097027	molecular_function	ubiquitin-protein transferase activator activity
D	0098793	cellular_component	presynapse
D	1903955	biological_process	positive regulation of protein targeting to mitochondrion
H	0000151	cellular_component	ubiquitin ligase complex
H	0000209	biological_process	protein polyubiquitination
H	0004842	molecular_function	ubiquitin-protein transferase activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0008270	molecular_function	zinc ion binding
H	0015030	cellular_component	Cajal body
H	0016567	biological_process	protein ubiquitination
H	0016604	cellular_component	nuclear body
H	0016740	molecular_function	transferase activity
H	0019005	cellular_component	SCF ubiquitin ligase complex
H	0019787	molecular_function	ubiquitin-like protein transferase activity
H	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
H	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
H	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
H	0031624	molecular_function	ubiquitin conjugating enzyme binding
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0039585	biological_process	PKR/eIFalpha signaling
H	0046872	molecular_function	metal ion binding
H	0061630	molecular_function	ubiquitin protein ligase activity
H	0097413	cellular_component	Lewy body
K	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
K	0005515	molecular_function	protein binding
K	0005654	cellular_component	nucleoplasm
K	0006355	biological_process	regulation of DNA-templated transcription
K	0007346	biological_process	regulation of mitotic cell cycle
K	0016538	molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity
K	0019005	cellular_component	SCF ubiquitin ligase complex
K	0019901	molecular_function	protein kinase binding
K	0042393	molecular_function	histone binding
K	0043130	molecular_function	ubiquitin binding
K	0044772	biological_process	mitotic cell cycle phase transition
K	0048144	biological_process	fibroblast proliferation
K	0051301	biological_process	cell division
K	0061575	molecular_function	cyclin-dependent protein serine/threonine kinase activator activity
L	0000082	biological_process	G1/S transition of mitotic cell cycle
L	0000086	biological_process	G2/M transition of mitotic cell cycle
L	0000122	biological_process	negative regulation of transcription by RNA polymerase II
L	0000287	molecular_function	magnesium ion binding
L	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
L	0000781	cellular_component	chromosome, telomeric region
L	0000793	cellular_component	condensed chromosome
L	0000805	cellular_component	X chromosome
L	0000806	cellular_component	Y chromosome
L	0001673	cellular_component	male germ cell nucleus
L	0004672	molecular_function	protein kinase activity
L	0004674	molecular_function	protein serine/threonine kinase activity
L	0004693	molecular_function	cyclin-dependent protein serine/threonine kinase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0005635	cellular_component	nuclear envelope
L	0005654	cellular_component	nucleoplasm
L	0005667	cellular_component	transcription regulator complex
L	0005737	cellular_component	cytoplasm
L	0005768	cellular_component	endosome
L	0005813	cellular_component	centrosome
L	0005829	cellular_component	cytosol
L	0005856	cellular_component	cytoskeleton
L	0006260	biological_process	DNA replication
L	0006281	biological_process	DNA repair
L	0006338	biological_process	chromatin remodeling
L	0006351	biological_process	DNA-templated transcription
L	0006468	biological_process	protein phosphorylation
L	0006813	biological_process	potassium ion transport
L	0007099	biological_process	centriole replication
L	0007165	biological_process	signal transduction
L	0007265	biological_process	Ras protein signal transduction
L	0007346	biological_process	regulation of mitotic cell cycle
L	0008284	biological_process	positive regulation of cell population proliferation
L	0010389	biological_process	regulation of G2/M transition of mitotic cell cycle
L	0010468	biological_process	regulation of gene expression
L	0015030	cellular_component	Cajal body
L	0016301	molecular_function	kinase activity
L	0018105	biological_process	peptidyl-serine phosphorylation
L	0019904	molecular_function	protein domain specific binding
L	0030332	molecular_function	cyclin binding
L	0031453	biological_process	positive regulation of heterochromatin formation
L	0031571	biological_process	mitotic G1 DNA damage checkpoint signaling
L	0032298	biological_process	positive regulation of DNA-templated DNA replication initiation
L	0035173	molecular_function	histone kinase activity
L	0043247	biological_process	telomere maintenance in response to DNA damage
L	0043687	biological_process	post-translational protein modification
L	0045740	biological_process	positive regulation of DNA replication
L	0045893	biological_process	positive regulation of DNA-templated transcription
L	0046872	molecular_function	metal ion binding
L	0051298	biological_process	centrosome duplication
L	0051301	biological_process	cell division
L	0051321	biological_process	meiotic cell cycle
L	0071732	biological_process	cellular response to nitric oxide
L	0090398	biological_process	cellular senescence
L	0097123	cellular_component	cyclin A1-CDK2 complex
L	0097124	cellular_component	cyclin A2-CDK2 complex
L	0097134	cellular_component	cyclin E1-CDK2 complex
L	0097135	cellular_component	cyclin E2-CDK2 complex
L	0097472	molecular_function	cyclin-dependent protein kinase activity
L	0106310	molecular_function	protein serine kinase activity
L	1905784	biological_process	regulation of anaphase-promoting complex-dependent catabolic process
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005829	cellular_component	cytosol
N	0006357	biological_process	regulation of transcription by RNA polymerase II
N	0006508	biological_process	proteolysis
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
N	0008104	biological_process	protein localization
N	0009653	biological_process	anatomical structure morphogenesis
N	0016567	biological_process	protein ubiquitination
N	0019941	biological_process	modification-dependent protein catabolic process
N	0030162	biological_process	regulation of proteolysis
N	0031386	molecular_function	protein tag activity
N	0031625	molecular_function	ubiquitin protein ligase binding
N	0036211	biological_process	protein modification process
N	0045116	biological_process	protein neddylation
N	0070062	cellular_component	extracellular exosome
P	0000079	biological_process	regulation of cyclin-dependent protein serine/threonine kinase activity
P	0000082	biological_process	G1/S transition of mitotic cell cycle
P	0001666	biological_process	response to hypoxia
P	0001890	biological_process	placenta development
P	0001934	biological_process	positive regulation of protein phosphorylation
P	0004860	molecular_function	protein kinase inhibitor activity
P	0004861	molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005768	cellular_component	endosome
P	0005829	cellular_component	cytosol
P	0006813	biological_process	potassium ion transport
P	0006915	biological_process	apoptotic process
P	0006977	biological_process	DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
P	0007096	biological_process	regulation of exit from mitosis
P	0007219	biological_process	Notch signaling pathway
P	0007346	biological_process	regulation of mitotic cell cycle
P	0007507	biological_process	heart development
P	0007605	biological_process	sensory perception of sound
P	0008284	biological_process	positive regulation of cell population proliferation
P	0008285	biological_process	negative regulation of cell population proliferation
P	0009410	biological_process	response to xenobiotic stimulus
P	0009749	biological_process	response to glucose
P	0014070	biological_process	response to organic cyclic compound
P	0019901	molecular_function	protein kinase binding
P	0019903	molecular_function	protein phosphatase binding
P	0019914	molecular_function	cyclin-dependent protein kinase activating kinase regulator activity
P	0030308	biological_process	negative regulation of cell growth
P	0030332	molecular_function	cyclin binding
P	0030334	biological_process	regulation of cell migration
P	0030544	molecular_function	Hsp70 protein binding
P	0031116	biological_process	positive regulation of microtubule polymerization
P	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
P	0031625	molecular_function	ubiquitin protein ligase binding
P	0032355	biological_process	response to estradiol
P	0032991	cellular_component	protein-containing complex
P	0033673	biological_process	negative regulation of kinase activity
P	0042326	biological_process	negative regulation of phosphorylation
P	0043200	biological_process	response to amino acid
P	0043231	cellular_component	intracellular membrane-bounded organelle
P	0043434	biological_process	response to peptide hormone
P	0044877	molecular_function	protein-containing complex binding
P	0045732	biological_process	positive regulation of protein catabolic process
P	0045736	biological_process	negative regulation of cyclin-dependent protein serine/threonine kinase activity
P	0045740	biological_process	positive regulation of DNA replication
P	0045892	biological_process	negative regulation of DNA-templated transcription
P	0045930	biological_process	negative regulation of mitotic cell cycle
P	0046686	biological_process	response to cadmium ion
P	0048102	biological_process	autophagic cell death
P	0048839	biological_process	inner ear development
P	0050680	biological_process	negative regulation of epithelial cell proliferation
P	0051087	molecular_function	protein-folding chaperone binding
P	0051168	biological_process	nuclear export
P	0051726	biological_process	regulation of cell cycle
P	0060090	molecular_function	molecular adaptor activity
P	0060767	biological_process	epithelial cell proliferation involved in prostate gland development
P	0060770	biological_process	negative regulation of epithelial cell proliferation involved in prostate gland development
P	0071236	biological_process	cellular response to antibiotic
P	0071285	biological_process	cellular response to lithium ion
P	0071407	biological_process	cellular response to organic cyclic compound
P	0071456	biological_process	cellular response to hypoxia
P	0090398	biological_process	cellular senescence
P	0140678	molecular_function	molecular function inhibitor activity
P	1902746	biological_process	regulation of lens fiber cell differentiation
P	1902806	biological_process	regulation of cell cycle G1/S phase transition
P	1904019	biological_process	epithelial cell apoptotic process
P	1904030	biological_process	negative regulation of cyclin-dependent protein kinase activity
P	1904036	biological_process	negative regulation of epithelial cell apoptotic process
P	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
P	1905179	biological_process	negative regulation of cardiac muscle tissue regeneration
P	1990757	molecular_function	ubiquitin ligase activator activity
P	2000045	biological_process	regulation of G1/S transition of mitotic cell cycle
R	0000165	biological_process	MAPK cascade
R	0000209	biological_process	protein polyubiquitination
R	0004842	molecular_function	ubiquitin-protein transferase activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006281	biological_process	DNA repair
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0006513	biological_process	protein monoubiquitination
R	0006974	biological_process	DNA damage response
R	0007283	biological_process	spermatogenesis
R	0008270	molecular_function	zinc ion binding
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0019005	cellular_component	SCF ubiquitin ligase complex
R	0019788	molecular_function	NEDD8 transferase activity
R	0030163	biological_process	protein catabolic process
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031461	cellular_component	cullin-RING ubiquitin ligase complex
R	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
R	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
R	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
R	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
R	0031625	molecular_function	ubiquitin protein ligase binding
R	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032480	biological_process	negative regulation of type I interferon production
R	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
R	0034644	biological_process	cellular response to UV
R	0042110	biological_process	T cell activation
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0043687	biological_process	post-translational protein modification
R	0045116	biological_process	protein neddylation
R	0045732	biological_process	positive regulation of protein catabolic process
R	0046872	molecular_function	metal ion binding
R	0060090	molecular_function	molecular adaptor activity
R	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0062197	biological_process	cellular response to chemical stress
R	0070936	biological_process	protein K48-linked ubiquitination
R	0071230	biological_process	cellular response to amino acid stimulus
R	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
R	0097602	molecular_function	cullin family protein binding
R	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R	1900076	biological_process	regulation of cellular response to insulin stimulus
R	1902499	biological_process	positive regulation of protein autoubiquitination
R	1902883	biological_process	negative regulation of response to oxidative stress
R	1904263	biological_process	positive regulation of TORC1 signaling
S	0000209	biological_process	protein polyubiquitination
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005829	cellular_component	cytosol
S	0006338	biological_process	chromatin remodeling
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0006513	biological_process	protein monoubiquitination
S	0008013	molecular_function	beta-catenin binding
S	0016567	biological_process	protein ubiquitination
S	0019005	cellular_component	SCF ubiquitin ligase complex
S	0019904	molecular_function	protein domain specific binding
S	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
S	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
S	0031519	cellular_component	PcG protein complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051457	biological_process	maintenance of protein location in nucleus
S	0070936	biological_process	protein K48-linked ubiquitination
S	0097602	molecular_function	cullin family protein binding
S	0140677	molecular_function	molecular function activator activity
S	0160072	molecular_function	ubiquitin ligase complex scaffold activity
S	1904668	biological_process	positive regulation of ubiquitin protein ligase activity
S	1990444	molecular_function	F-box domain binding
S	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
T	0000082	biological_process	G1/S transition of mitotic cell cycle
T	0000086	biological_process	G2/M transition of mitotic cell cycle
T	0000209	biological_process	protein polyubiquitination
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005730	cellular_component	nucleolus
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0016567	biological_process	protein ubiquitination
T	0019005	cellular_component	SCF ubiquitin ligase complex
T	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
T	0033148	biological_process	positive regulation of intracellular estrogen receptor signaling pathway
T	0042802	molecular_function	identical protein binding
T	0042981	biological_process	regulation of apoptotic process
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0045087	biological_process	innate immune response
T	0051607	biological_process	defense response to virus
T	0051726	biological_process	regulation of cell cycle
T	0070534	biological_process	protein K63-linked ubiquitination
T	0070936	biological_process	protein K48-linked ubiquitination
T	1902916	biological_process	positive regulation of protein polyubiquitination
T	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
T	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
Y	0000082	biological_process	G1/S transition of mitotic cell cycle
Y	0000086	biological_process	G2/M transition of mitotic cell cycle
Y	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
Y	0001939	cellular_component	female pronucleus
Y	0001940	cellular_component	male pronucleus
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005813	cellular_component	centrosome
Y	0005829	cellular_component	cytosol
Y	0006275	biological_process	regulation of DNA replication
Y	0006351	biological_process	DNA-templated transcription
Y	0007265	biological_process	Ras protein signal transduction
Y	0016538	molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity
Y	0019901	molecular_function	protein kinase binding
Y	0019904	molecular_function	protein domain specific binding
Y	0031100	biological_process	animal organ regeneration
Y	0032355	biological_process	response to estradiol
Y	0033762	biological_process	response to glucagon
Y	0036120	biological_process	cellular response to platelet-derived growth factor stimulus
Y	0043687	biological_process	post-translational protein modification
Y	0044320	biological_process	cellular response to leptin stimulus
Y	0044772	biological_process	mitotic cell cycle phase transition
Y	0044843	biological_process	cell cycle G1/S phase transition
Y	0045893	biological_process	positive regulation of DNA-templated transcription
Y	0048146	biological_process	positive regulation of fibroblast proliferation
Y	0051301	biological_process	cell division
Y	0071314	biological_process	cellular response to cocaine
Y	0071373	biological_process	cellular response to luteinizing hormone stimulus
Y	0071392	biological_process	cellular response to estradiol stimulus
Y	0071407	biological_process	cellular response to organic cyclic compound
Y	0071456	biological_process	cellular response to hypoxia
Y	0071732	biological_process	cellular response to nitric oxide
Y	0090102	biological_process	cochlea development
Y	0097122	cellular_component	cyclin A2-CDK1 complex
Y	0097124	cellular_component	cyclin A2-CDK2 complex
Y	1990314	biological_process	cellular response to insulin-like growth factor stimulus
Y	2000573	biological_process	positive regulation of DNA biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGEGTYGVVYkArnkltgev..........VALK

Chain	Residue	Details
L	ILE10-LYS33

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VlHrDLKpqNLLI

Chain	Residue	Details
L	VAL123-ILE135

---

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNIdek.GqVCLpvI

Chain	Residue	Details
D	TYR75-ILE90

---

site_id	PS00292
Number of Residues	32
Details	CYCLINS Cyclins signature. RaiLvdWLvevgeeykLqnetLhlAVnYIDRF

Chain	Residue	Details
Y	ARG211-PHE242

---

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KerVeEkegIPpqqQrLIYsGkqmnD

Chain	Residue	Details
N	LYS27-ASP52
U	LYS27-ASP52

---

site_id	PS00944
Number of Residues	19
Details	CKS_1 Cyclin-dependent kinases regulatory subunits signature 1. YSdKYdDEeFEYRHVmLPK

Chain	Residue	Details
K	TYR8-LYS26

---

site_id	PS00945
Number of Residues	11
Details	CKS_2 Cyclin-dependent kinases regulatory subunits signature 2. HePEpHILLFR

Chain	Residue	Details
K	HIS60-ARG70

---

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIdiLIEKeYLeRvdgekdtYsYlA

Chain	Residue	Details
C	ILE749-ALA776

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133

Chain	Residue	Details
D	CYS86
N	ILE44

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
D	LYS131
R	CYS94
R	ASP97
R	CYS45
R	CYS53
R	CYS56
R	CYS68
R	CYS75
R	HIS77
R	HIS80
R	HIS82

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O

Chain	Residue	Details
R	CYS83
T	SER75

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
R	MET1
L	LYS88
L	LEU166

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
R	ALA2
H	CYS281
H	CYS297
H	CYS299
H	CYS304
H	CYS307
H	HIS312
H	CYS317
H	CYS189
H	CYS203
H	HIS205
H	CYS208
H	CYS211
H	CYS231
H	CYS236
H	CYS276

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
R	THR9
U	LYS63
H	CYS362
H	CYS367

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site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
U	LYS11
U	LYS48
H	HIS382
H	CYS389

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
U	LYS27

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site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
U	LYS29

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site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
U	LYS33

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
D	CYS86	nucleofuge

---