7B51
Crystal structure of human CRM1 covalently modified by 2-mercaptoethanol at Cys528
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | GTP-binding nuclear protein Ran | polymer | 182 | 20782.2 | 1 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (Human) | Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein |
| 2 | B (A) | Exportin-1 | polymer | 1060 | 121966.5 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Homo sapiens (Human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 3 | C (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 4 | D (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (A) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 1 | Chemie (BME) | |||
| 6 | F, G (B, A) | water | water | 18.0 | 136 | Chemie (HOH) |
Sequence modifications
B: 1 - 180 (UniProt: P62826)
A: 1 - 1036 (UniProt: O14980)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Gly 0 | - | expression tag |
| Leu 69 | Gln 69 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Ala -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Met -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Met -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 430 | Val 430 | engineered mutation |
| Ala 431 | Leu 431 | engineered mutation |
| Ala 432 | Val 432 | engineered mutation |
| Arg 1037 | - | expression tag |
| Ser 1038 | - | expression tag |
| Arg 1039 | - | expression tag |
| Ser 1040 | - | expression tag |
| His 1041 | - | expression tag |
| His 1042 | - | expression tag |
| His 1043 | - | expression tag |
| His 1044 | - | expression tag |
| His 1045 | - | expression tag |
| His 1046 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 142748.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 625.6 | |
| All* | Total formula weight | 143374.4 |
