7AUD
Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Bloom syndrome protein,Bloom syndrome protein | polymer | 563 | 63941.5 | 6 | UniProt (P54132) Pfam (PF00270) Pfam (PF00271) Pfam (PF16124) Pfam (PF00570) | Homo sapiens (Human) | DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3,DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3 |
| 2 | G, H, I, J, K... (M, N, O, P, Q...) | DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3') | polymer | 15 | 4576.0 | 6 | synthetic construct | ||
| 3 | AA, EA, IA, M, R... (D, E, F, A, B...) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 4 | BA, FA, JA, N, S... (D, E, F, A, B...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 6 | Chemie (ADP) | |||
| 5 | CA, GA, KA, O, T... (D, E, F, A, B...) | N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide | non-polymer | 401.5 | 6 | Chemie (RY8) | |||
| 6 | DA, HA, P, Q, U... (D, E, A, B, C) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 7 | Chemie (PG4) | |||
| 7 | LA, MA, NA, OA, PA... (F, M, N, P, Q) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 8 | RA, SA, TA, UA, VA... (A, B, C, D, E...) | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 636 - 1070 (UniProt: P54132)
A, B, C, D, E, F: 1202 - 1298 (UniProt: P54132)
| PDB | External Database | Details |
|---|---|---|
| Met 610 | - | initiating methionine |
| Gly 611 | - | expression tag |
| Ser 612 | - | expression tag |
| Ala 613 | - | expression tag |
| Trp 614 | - | expression tag |
| Ser 615 | - | expression tag |
| His 616 | - | expression tag |
| Pro 617 | - | expression tag |
| Gln 618 | - | expression tag |
| Phe 619 | - | expression tag |
| Glu 620 | - | expression tag |
| Lys 621 | - | expression tag |
| Ser 622 | - | expression tag |
| Ser 623 | - | expression tag |
| Gly 624 | - | expression tag |
| Leu 625 | - | expression tag |
| Glu 626 | - | expression tag |
| Val 627 | - | expression tag |
| Leu 628 | - | expression tag |
| Phe 629 | - | expression tag |
| Gln 630 | - | expression tag |
| Gly 631 | - | expression tag |
| Gly 632 | - | expression tag |
| Pro 633 | - | expression tag |
| His 634 | - | expression tag |
| Met 635 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1197 | - | linker |
| Ser 1198 | - | linker |
| Gly 1199 | - | linker |
| Gly 1200 | - | linker |
| Ser 1201 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 411104.9 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 6870.1 | |
| All* | Total formula weight | 417975.0 |
