7AUD

Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Bloom syndrome protein,Bloom syndrome protein	polymer	563	63941.5	6	UniProt (P54132) Pfam (PF00270) Pfam (PF00271) Pfam (PF16124) Pfam (PF00570) In PDB	Homo sapiens (Human)	DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3,DNA helicase,RecQ-like type 2,RecQ2,RecQ protein-like 3
2	M, N, O, P, Q...	DNA (5'-D(*GP*TP*AP*CP*CP*CP*GP*AP*TP*GP*TP*GP*T)-3')	polymer	15	4576.0	6		synthetic construct
3	D, E, F, A, B...	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
4	D, E, F, A, B...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	6	Chemie (ADP)
5	D, E, F, A, B...	N-(2,3-dimethyl-5-sulfamoylphenyl)-4-(2-methylthiazol-4-yl)benzamide	non-polymer		401.5	6	Chemie (RY8)
6	D, E, A, B, C	TETRAETHYLENE GLYCOL	non-polymer		194.2	7	Chemie (PG4)
7	F, M, N, P, Q	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
8		water	water		18.0	66	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 636 - 1070 (UniProt: P54132)

PDB	External Database	Details
Met 610	-	initiating methionine
Gly 611	-	expression tag
Ser 612	-	expression tag
Ala 613	-	expression tag
Trp 614	-	expression tag
Ser 615	-	expression tag
His 616	-	expression tag
Pro 617	-	expression tag
Gln 618	-	expression tag
Phe 619	-	expression tag
Glu 620	-	expression tag
Lys 621	-	expression tag
Ser 622	-	expression tag
Ser 623	-	expression tag
Gly 624	-	expression tag
Leu 625	-	expression tag
Glu 626	-	expression tag
Val 627	-	expression tag
Leu 628	-	expression tag
Phe 629	-	expression tag
Gln 630	-	expression tag
Gly 631	-	expression tag
Gly 632	-	expression tag
Pro 633	-	expression tag
His 634	-	expression tag
Met 635	-	expression tag

A, B, C, D, E, F: 1202 - 1298 (UniProt: P54132)

PDB	External Database	Details
Gly 1197	-	linker
Ser 1198	-	linker
Gly 1199	-	linker
Gly 1200	-	linker
Ser 1201	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	411104.9
Non-Polymers*	Number of molecules	31
	Total formula weight	6870.1
All*	Total formula weight	417975.0

*Water molecules are not included.