7AUD
Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0004386 | molecular_function | helicase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006310 | biological_process | DNA recombination |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0004386 | molecular_function | helicase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006310 | biological_process | DNA recombination |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0003676 | molecular_function | nucleic acid binding |
| C | 0004386 | molecular_function | helicase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0006310 | biological_process | DNA recombination |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0003676 | molecular_function | nucleic acid binding |
| D | 0004386 | molecular_function | helicase activity |
| D | 0005524 | molecular_function | ATP binding |
| D | 0006310 | biological_process | DNA recombination |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0003676 | molecular_function | nucleic acid binding |
| E | 0004386 | molecular_function | helicase activity |
| E | 0005524 | molecular_function | ATP binding |
| E | 0006310 | biological_process | DNA recombination |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0003676 | molecular_function | nucleic acid binding |
| F | 0004386 | molecular_function | helicase activity |
| F | 0005524 | molecular_function | ATP binding |
| F | 0006310 | biological_process | DNA recombination |
Functional Information from PROSITE/UniProt
| site_id | PS00690 |
| Number of Residues | 10 |
| Details | DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ArFVIDEAHC |
| Chain | Residue | Details |
| A | ALA790-CYS799 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1050 |
| Details | Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 882 |
| Details | Domain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | Region: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 30 |
| Details | Region: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 102 |
| Details | Region: {"description":"Necessary for ssDNA and DNA Holliday junction binding","evidences":[{"source":"PubMed","id":"20639533","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 18 |
| Details | Motif: {"description":"DEAH box"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 72 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CDG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CDG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 12 |
| Details | Site: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 6 |
| Details | Site: {"description":"3' overhang DNA-binding; via amide nitrogen","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 12 |
| Details | Site: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 6 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
