7AUD
Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97623 |
Spacegroup name | P 1 |
Unit cell lengths | 84.916, 111.709, 132.344 |
Unit cell angles | 72.64, 80.18, 79.22 |
Refinement procedure
Resolution | 47.280 - 2.960 |
R-factor | 0.2311 |
Rwork | 0.229 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3m |
Data reduction software | Aimless |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.280 | 3.070 |
High resolution limit [Å] | 2.960 | 2.970 |
Number of reflections | 91662 | 8892 |
<I/σ(I)> | 7.8 | 1.4 |
Completeness [%] | 98.0 | 94.5 |
Redundancy | 2.6 | |
CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | Morpheus HT-96, Condition C9, Molecular Dimensions. 0.09 M NPS, 0.1M Buffer System, 30% Precipitant Mix 1 NPS = 0.3 M sodium nitrate, 0.3 M sodium phosphate dibasic, 0.3 M ammonium sulphate Buffer System 1 = 1.0 M imidazole, MES monohydrate (acid) pH 6.5 60% Precipitant Mix 1 = 40% v/v PEG 500 MME, 20% w/v PEG 20000 |