6WIH
N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cysteine desulfurase, mitochondrial | polymer | 406 | 45081.6 | 1 | UniProt (Q9Y697) Pfam (PF00266) | Homo sapiens (Human) | |
| 2 | B (B) | LYR motif-containing protein 4 | polymer | 91 | 10763.5 | 1 | UniProt (Q9HD34) Pfam (PF05347) | Homo sapiens (Human) | |
| 3 | C (C) | Acyl carrier protein | polymer | 77 | 8514.3 | 1 | UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8) | Escherichia coli | ACP |
| 4 | D (D) | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial | polymer | 143 | 15605.0 | 1 | UniProt (Q9H1K1) Pfam (PF01592) | Homo sapiens (Human) | NifU-like N-terminal domain-containing protein,NifU-like protein |
| 5 | E (A) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 6 | AB, BA, EA, F, RA... (B, A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 9 | Chemie (PEG) | |||
| 7 | G, H, I, MB, VA (A, D, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 8 | BB, CA, CB, DA, DB... (B, A, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 38 | Chemie (EDO) | |||
| 9 | U, V (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
| 10 | AA (A) | 2,5,8,11,14,17-HEXAOXANONADECAN-19-OL | non-polymer | 296.4 | 1 | Chemie (P15) | |||
| 11 | FA, NA, OA (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
| 12 | EB (B) | {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID | non-polymer | 292.2 | 1 | Chemie (EDT) | |||
| 13 | FB (C) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 1 | Chemie (8Q1) | |||
| 14 | KB (D) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 15 | OB, PB, QB, RB (A, B, C, D) | water | water | 18.0 | 431 | Chemie (HOH) |
Sequence modifications
A: 56 - 457 (UniProt: Q9Y697)
B: 1 - 91 (UniProt: Q9HD34)
D: 35 - 167 (UniProt: Q9H1K1)
| PDB | External Database | Details |
|---|---|---|
| Met 52 | - | initiating methionine |
| Gly 53 | - | expression tag |
| Ser 54 | - | expression tag |
| Ser 55 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 11 | Ser 11 | variant |
| PDB | External Database | Details |
|---|---|---|
| Met 33 | - | initiating methionine |
| Ala 34 | - | expression tag |
| His 36 | Ser 11 | engineered mutation |
| Leu 168 | - | expression tag |
| Glu 169 | - | expression tag |
| His 170 | - | expression tag |
| His 171 | - | expression tag |
| His 172 | - | expression tag |
| His 173 | - | expression tag |
| His 174 | - | expression tag |
| His 175 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 79964.3 | |
| Non-Polymers* | Number of molecules | 62 |
| Total formula weight | 6227.8 | |
| All* | Total formula weight | 86192.1 |
