6WIH
N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Cysteine desulfurase, mitochondrial | polymer | 406 | 45081.6 | 1 | UniProt (Q9Y697) Pfam (PF00266) In PDB | Homo sapiens (Human) | |
2 | B | LYR motif-containing protein 4 | polymer | 91 | 10763.5 | 1 | UniProt (Q9HD34) Pfam (PF05347) In PDB | Homo sapiens (Human) | |
3 | C | Acyl carrier protein | polymer | 77 | 8514.3 | 1 | UniProt (B7MJ81) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8) In PDB | Escherichia coli | ACP |
4 | D | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial | polymer | 143 | 15605.0 | 1 | UniProt (Q9H1K1) Pfam (PF01592) In PDB | Homo sapiens (Human) | NifU-like N-terminal domain-containing protein,NifU-like protein |
5 | A | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
6 | B, A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 9 | Chemie (PEG) | |||
7 | A, D, B | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
8 | B, A, C, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 38 | Chemie (EDO) | |||
9 | A | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 2 | Chemie (PG4) | |||
10 | A | 2,5,8,11,14,17-HEXAOXANONADECAN-19-OL | non-polymer | 296.4 | 1 | Chemie (P15) | |||
11 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
12 | B | {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID | non-polymer | 292.2 | 1 | Chemie (EDT) | |||
13 | C | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 1 | Chemie (8Q1) | |||
14 | D | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
15 | water | water | 18.0 | 431 | Chemie (HOH) |
Sequence modifications
A: 56 - 457 (UniProt: Q9Y697)
B: 1 - 91 (UniProt: Q9HD34)
D: 35 - 167 (UniProt: Q9H1K1)
PDB | External Database | Details |
---|---|---|
Met 52 | - | initiating methionine |
Gly 53 | - | expression tag |
Ser 54 | - | expression tag |
Ser 55 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 11 | Ser 11 | variant |
PDB | External Database | Details |
---|---|---|
Met 33 | - | initiating methionine |
Ala 34 | - | expression tag |
His 36 | Ser 11 | engineered mutation |
Leu 168 | - | expression tag |
Glu 169 | - | expression tag |
His 170 | - | expression tag |
His 171 | - | expression tag |
His 172 | - | expression tag |
His 173 | - | expression tag |
His 174 | - | expression tag |
His 175 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 79964.3 | |
Non-Polymers* | Number of molecules | 62 |
Total formula weight | 6227.8 | |
All* | Total formula weight | 86192.1 |