6WIH
N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0030170 | molecular_function | pyridoxal phosphate binding |
| A | 0031071 | molecular_function | cysteine desulfurase activity |
| A | 0044571 | biological_process | [2Fe-2S] cluster assembly |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0016226 | biological_process | iron-sulfur cluster assembly |
| B | 0016604 | cellular_component | nuclear body |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0044571 | biological_process | [2Fe-2S] cluster assembly |
| B | 0044572 | biological_process | [4Fe-4S] cluster assembly |
| B | 0099128 | cellular_component | mitochondrial iron-sulfur cluster assembly complex |
| B | 1990221 | cellular_component | L-cysteine desulfurase complex |
| B | 1990229 | cellular_component | iron-sulfur cluster assembly complex |
| C | 0000035 | molecular_function | acyl binding |
| C | 0000036 | molecular_function | acyl carrier activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006633 | biological_process | fatty acid biosynthetic process |
| C | 0008289 | molecular_function | lipid binding |
| C | 0008610 | biological_process | lipid biosynthetic process |
| C | 0009245 | biological_process | lipid A biosynthetic process |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0031177 | molecular_function | phosphopantetheine binding |
| D | 0005506 | molecular_function | iron ion binding |
| D | 0016226 | biological_process | iron-sulfur cluster assembly |
| D | 0051536 | molecular_function | iron-sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue PLP A 501 |
| Chain | Residue |
| A | GLY126 |
| A | SER255 |
| A | HIS257 |
| A | LYS258 |
| A | THR295 |
| A | HOH657 |
| A | HOH757 |
| A | ALA127 |
| A | THR128 |
| A | HIS156 |
| A | MET203 |
| A | ASN207 |
| A | ASP232 |
| A | ALA234 |
| A | GLN235 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue PEG A 502 |
| Chain | Residue |
| A | ILE270 |
| A | ARG271 |
| A | ARG275 |
| A | VAL276 |
| A | VAL278 |
| A | MET291 |
| A | HOH612 |
| A | HOH624 |
| site_id | AC3 |
| Number of Residues | 11 |
| Details | binding site for residue GOL A 503 |
| Chain | Residue |
| A | PRO58 |
| A | THR92 |
| A | HIS93 |
| A | ALA94 |
| A | TRP97 |
| A | LEU367 |
| A | MET368 |
| A | LEU370 |
| A | LYS371 |
| A | EDO509 |
| A | HOH804 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 504 |
| Chain | Residue |
| A | LYS431 |
| A | ARG434 |
| A | GLU435 |
| A | TRP440 |
| A | HOH638 |
| A | HOH666 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 505 |
| Chain | Residue |
| A | GLU190 |
| A | ARG220 |
| A | ILE221 |
| A | SER224 |
| A | ARG225 |
| A | HOH644 |
| A | HOH646 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 506 |
| Chain | Residue |
| A | GLN179 |
| A | ASP185 |
| A | LYS371 |
| A | ARG432 |
| A | EDO507 |
| A | EDO526 |
| D | GLU44 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 507 |
| Chain | Residue |
| A | MET368 |
| A | ALA369 |
| A | LYS371 |
| A | HIS429 |
| A | EDO506 |
| D | VAL40 |
| D | TYR43 |
| D | GLU44 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 508 |
| Chain | Residue |
| A | MET334 |
| A | LYS335 |
| A | LEU337 |
| A | PRO338 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 509 |
| Chain | Residue |
| A | TRP97 |
| A | LYS371 |
| A | GOL503 |
| A | HOH676 |
| A | HOH685 |
| A | HOH752 |
| D | LEU35 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 510 |
| Chain | Residue |
| A | LYS157 |
| A | PEG524 |
| A | HOH714 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 511 |
| Chain | Residue |
| A | GLU100 |
| A | ALA101 |
| A | HOH614 |
| A | HOH619 |
| A | HOH838 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 512 |
| Chain | Residue |
| A | LYS248 |
| A | ASP250 |
| A | ARG271 |
| A | HOH656 |
| A | HOH722 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 513 |
| Chain | Residue |
| A | SER125 |
| A | ARG292 |
| A | THR295 |
| A | PRO297 |
| A | HOH657 |
| A | HOH672 |
| A | HOH672 |
| A | HOH677 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 514 |
| Chain | Residue |
| A | GLU316 |
| A | EDO525 |
| A | HOH778 |
| B | PEG405 |
| site_id | AD6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 515 |
| Chain | Residue |
| A | SER181 |
| A | ILE183 |
| A | ASP345 |
| A | HOH610 |
| A | HOH618 |
| D | ASN45 |
| D | HOH312 |
| A | GLN179 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue PEG A 516 |
| Chain | Residue |
| A | ASP75 |
| A | HOH642 |
| A | HOH879 |
| B | ARG68 |
| B | LEU75 |
| B | SER77 |
| site_id | AD8 |
| Number of Residues | 10 |
| Details | binding site for residue PG4 A 517 |
| Chain | Residue |
| A | SER112 |
| A | GLY115 |
| A | ALA116 |
| A | GLN312 |
| A | GLN313 |
| A | EDO522 |
| A | PEG541 |
| A | HOH630 |
| A | HOH691 |
| A | HOH754 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue PG4 A 518 |
| Chain | Residue |
| A | ARG328 |
| A | ASN332 |
| A | LYS335 |
| A | ILE427 |
| A | HOH603 |
| A | HOH711 |
| site_id | AE1 |
| Number of Residues | 1 |
| Details | binding site for residue PEG A 519 |
| Chain | Residue |
| A | HIS319 |
| site_id | AE2 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 520 |
| Chain | Residue |
| A | ALA359 |
| A | TYR360 |
| A | ASP400 |
| A | LEU401 |
| A | SER404 |
| A | LEU439 |
| A | P15523 |
| D | PHE93 |
| site_id | AE3 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 521 |
| Chain | Residue |
| A | ARG119 |
| A | ARG272 |
| site_id | AE4 |
| Number of Residues | 2 |
| Details | binding site for residue EDO A 522 |
| Chain | Residue |
| A | GLN312 |
| A | PG4517 |
| site_id | AE5 |
| Number of Residues | 13 |
| Details | binding site for residue P15 A 523 |
| Chain | Residue |
| A | MET334 |
| A | LEU337 |
| A | PRO338 |
| A | ASP339 |
| A | VAL340 |
| A | VAL341 |
| A | ALA359 |
| A | TYR360 |
| A | ASP400 |
| A | LEU449 |
| A | EDO520 |
| A | HOH720 |
| A | HOH741 |
| site_id | AE6 |
| Number of Residues | 6 |
| Details | binding site for residue PEG A 524 |
| Chain | Residue |
| A | GLN153 |
| A | TYR175 |
| A | EDO510 |
| A | HOH714 |
| A | HOH766 |
| A | HOH843 |
| site_id | AE7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 525 |
| Chain | Residue |
| A | GLN313 |
| A | EDO514 |
| A | EDO538 |
| B | ARG35 |
| B | PEG405 |
| site_id | AE8 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 526 |
| Chain | Residue |
| A | LYS425 |
| A | GLN428 |
| A | HIS429 |
| A | EDO506 |
| A | HOH615 |
| site_id | AE9 |
| Number of Residues | 5 |
| Details | binding site for residue PEG A 527 |
| Chain | Residue |
| A | GLN331 |
| A | PRO346 |
| A | HOH628 |
| A | HOH641 |
| A | HOH801 |
| site_id | AF1 |
| Number of Residues | 11 |
| Details | binding site for residue PGE A 528 |
| Chain | Residue |
| A | HIS319 |
| A | SER323 |
| A | SER326 |
| A | ILE330 |
| A | PRO346 |
| A | HIS349 |
| A | PRO351 |
| A | EDO542 |
| A | HOH601 |
| A | HOH606 |
| A | HOH641 |
| site_id | AF2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 529 |
| Chain | Residue |
| A | GLU217 |
| A | HOH645 |
| A | HOH756 |
| A | HOH849 |
| D | ASN48 |
| site_id | AF3 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 530 |
| Chain | Residue |
| A | TYR421 |
| A | GLU424 |
| A | HOH792 |
| A | HOH839 |
| site_id | AF4 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 531 |
| Chain | Residue |
| A | HOH645 |
| A | HOH663 |
| A | HOH755 |
| A | HOH773 |
| site_id | AF5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 532 |
| Chain | Residue |
| A | LYS180 |
| A | ALA394 |
| A | GLY396 |
| A | HOH643 |
| A | HOH692 |
| site_id | AF6 |
| Number of Residues | 1 |
| Details | binding site for residue EDO A 533 |
| Chain | Residue |
| A | PHE141 |
| site_id | AF7 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 534 |
| Chain | Residue |
| A | GLN428 |
| A | ARG432 |
| A | HOH734 |
| A | HOH832 |
| site_id | AF8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 535 |
| Chain | Residue |
| A | PHE141 |
| A | TYR142 |
| A | ARG143 |
| A | SER144 |
| site_id | AF9 |
| Number of Residues | 10 |
| Details | binding site for residue PGE A 536 |
| Chain | Residue |
| A | ARG105 |
| A | GLN109 |
| A | ALA306 |
| A | GLU309 |
| A | GLN313 |
| A | HOH613 |
| A | HOH667 |
| A | HOH687 |
| B | ARG61 |
| B | HOH549 |
| site_id | AG1 |
| Number of Residues | 3 |
| Details | binding site for residue PGE A 537 |
| Chain | Residue |
| A | ARG105 |
| A | HOH850 |
| A | HOH889 |
| site_id | AG2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 538 |
| Chain | Residue |
| A | GLU314 |
| A | EDO525 |
| A | HOH710 |
| B | ARG35 |
| C | 8Q1301 |
| site_id | AG3 |
| Number of Residues | 4 |
| Details | binding site for residue EDO A 539 |
| Chain | Residue |
| A | GLN287 |
| A | ALA384 |
| A | HOH718 |
| A | HOH731 |
| site_id | AG4 |
| Number of Residues | 8 |
| Details | binding site for residue PEG A 540 |
| Chain | Residue |
| A | ASP161 |
| A | SER283 |
| A | GLY284 |
| A | GLY286 |
| A | GLY290 |
| A | HOH620 |
| A | HOH737 |
| A | HOH822 |
| site_id | AG5 |
| Number of Residues | 7 |
| Details | binding site for residue PEG A 541 |
| Chain | Residue |
| A | GLY115 |
| A | ALA116 |
| A | ASP117 |
| A | PG4517 |
| A | HOH691 |
| A | HOH705 |
| A | HOH836 |
| site_id | AG6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO A 542 |
| Chain | Residue |
| A | LYS212 |
| A | PRO346 |
| A | LYS347 |
| A | HIS348 |
| A | HIS349 |
| A | PGE528 |
| A | HOH606 |
| A | HOH773 |
| site_id | AG7 |
| Number of Residues | 3 |
| Details | binding site for residue EDO B 401 |
| Chain | Residue |
| B | ARG41 |
| B | LYS44 |
| C | ASP35 |
| site_id | AG8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL B 402 |
| Chain | Residue |
| A | ARG321 |
| A | GLU416 |
| A | HOH791 |
| B | ARG34 |
| site_id | AG9 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 403 |
| Chain | Residue |
| A | PRO68 |
| A | TYR85 |
| A | PHE413 |
| A | THR414 |
| A | HOH715 |
| B | ASN27 |
| B | HOH504 |
| site_id | AH1 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 404 |
| Chain | Residue |
| A | ASP445 |
| A | HOH855 |
| B | HOH505 |
| B | HOH529 |
| site_id | AH2 |
| Number of Residues | 8 |
| Details | binding site for residue PEG B 405 |
| Chain | Residue |
| A | TYR317 |
| A | EDO514 |
| A | EDO525 |
| B | ARG35 |
| B | ASP38 |
| B | ALA39 |
| B | GLU42 |
| B | HOH537 |
| site_id | AH3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 406 |
| Chain | Residue |
| A | ASP445 |
| A | GLY446 |
| B | ARG67 |
| B | PEG407 |
| B | EDO408 |
| site_id | AH4 |
| Number of Residues | 5 |
| Details | binding site for residue PEG B 407 |
| Chain | Residue |
| A | HOH642 |
| B | ARG68 |
| B | LEU75 |
| B | EDO406 |
| B | EDO408 |
| site_id | AH5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 408 |
| Chain | Residue |
| A | ASP445 |
| A | GLY446 |
| B | GLY64 |
| B | ARG67 |
| B | EDO406 |
| B | PEG407 |
| B | HOH502 |
| site_id | AH6 |
| Number of Residues | 2 |
| Details | binding site for residue EDO B 409 |
| Chain | Residue |
| B | GLU42 |
| B | ASN43 |
| site_id | AH7 |
| Number of Residues | 5 |
| Details | binding site for residue EDO B 410 |
| Chain | Residue |
| B | LYS21 |
| B | ARG22 |
| B | PHE23 |
| B | LYS80 |
| B | EDT411 |
| site_id | AH8 |
| Number of Residues | 7 |
| Details | binding site for residue EDT B 411 |
| Chain | Residue |
| A | GLU417 |
| B | LYS21 |
| B | ARG29 |
| B | ILE83 |
| B | GLU84 |
| B | EDO410 |
| B | HOH518 |
| site_id | AH9 |
| Number of Residues | 12 |
| Details | binding site for residue 8Q1 C 301 |
| Chain | Residue |
| A | EDO538 |
| B | ARG6 |
| B | ALA39 |
| B | PHE40 |
| B | ASN43 |
| B | LYS44 |
| B | VAL46 |
| B | HOH514 |
| C | ASP35 |
| C | SER36 |
| C | EDO302 |
| C | HOH414 |
| site_id | AI1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 302 |
| Chain | Residue |
| C | VAL29 |
| C | ASP35 |
| C | SER36 |
| C | 8Q1301 |
| C | HOH403 |
| site_id | AI2 |
| Number of Residues | 6 |
| Details | binding site for residue EDO C 303 |
| Chain | Residue |
| B | ARG37 |
| B | ARG41 |
| C | GLN14 |
| C | ASP38 |
| C | GLU41 |
| C | LEU42 |
| site_id | AI3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO C 304 |
| Chain | Residue |
| C | PRO55 |
| C | TYR71 |
| C | HIS75 |
| D | SER56 |
| D | LYS57 |
| site_id | AI4 |
| Number of Residues | 5 |
| Details | binding site for residue EDO D 201 |
| Chain | Residue |
| A | ARG220 |
| A | HOH841 |
| D | GLY50 |
| D | SER51 |
| D | ARG89 |
| site_id | AI5 |
| Number of Residues | 6 |
| Details | binding site for residue 1PE D 202 |
| Chain | Residue |
| D | GLY111 |
| D | LYS112 |
| D | GLU116 |
| D | THR119 |
| D | ILE120 |
| D | HOH304 |
| site_id | AI6 |
| Number of Residues | 2 |
| Details | binding site for residue EDO D 203 |
| Chain | Residue |
| D | THR61 |
| D | LEU63 |
| site_id | AI7 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 204 |
| Chain | Residue |
| B | ARG14 |
| C | ASP56 |
| D | ASP53 |
| D | THR55 |
| D | HOH310 |
| D | HOH314 |
| site_id | AI8 |
| Number of Residues | 2 |
| Details | binding site for residue EDO D 205 |
| Chain | Residue |
| D | TRP108 |
| D | HOH327 |
Functional Information from PROSITE/UniProt
| site_id | PS00595 |
| Number of Residues | 20 |
| Details | AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI |
| Chain | Residue | Details |
| A | ILE249-ILE268 |
| site_id | PS00012 |
| Number of Residues | 16 |
| Details | PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL |
| Chain | Residue | Details |
| C | ASP31-LEU46 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:Q9D7P6 |
| Chain | Residue | Details |
| B | LYS44 | |
| D | GLY94 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:O29689 |
| Chain | Residue | Details |
| D | GLU163 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:31101807, ECO:0007744|PDB:6NZU |
| Chain | Residue | Details |
| D | ILE120 | |
| D | GLU163 | |
| A | HIS257 | |
| D | GLY96 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | SITE: Mediates ISCU dimerization and de novo [2Fe-2S] cluster assembly => ECO:0000269|PubMed:34824239 |
| Chain | Residue | Details |
| D | GLY60 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by MTOR => ECO:0000269|PubMed:23508953 |
| Chain | Residue | Details |
| D | VAL39 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine persulfide => ECO:0000250|UniProtKB:Q9D7P6 |
| Chain | Residue | Details |
| D | GLY94 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine persulfide => ECO:0000305|PubMed:24971490 |
| Chain | Residue | Details |
| D | GLU163 |
