6WIH
N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.350, 86.350, 245.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.972 - 1.900 |
R-factor | 0.1523 |
Rwork | 0.151 |
R-free | 0.18610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6w1d |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.972 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 74322 | 7325 |
<I/σ(I)> | 17.66 | |
Completeness [%] | 100.0 | |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 288 | 0.1 M MES pH 5.5 22.5 % PEG 400 |