6WGE

Cryo-EM structure of human Cohesin-NIPBL-DNA complex without STAG1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Structural maintenance of chromosomes protein 1A	polymer	1233	143484.1	1	UniProt (Q14683) Pfam (PF02463) Pfam (PF06470)	Homo sapiens (Human)	SMC-1A,Sb1.8
2	B (B)	Structural maintenance of chromosomes protein 3	polymer	1217	141770.6	1	UniProt (Q9UQE7) Pfam (PF02463) Pfam (PF06470)	Homo sapiens (Human)	SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP
3	C (C)	Double-strand-break repair protein rad21 homolog	polymer	631	71556.1	1	UniProt (O60216) Pfam (PF04825) Pfam (PF04824)	Homo sapiens (Human)	hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog
4	D (E)	Nipped-B-like protein	polymer	1642	186778.3	1	UniProt (Q6KC79) Pfam (PF12765) Pfam (PF12830)	Homo sapiens (Human)	Delangin,SCC2 homolog
5	E (F)	DNA (43-MER)	polymer	43	13422.9	1		Homo sapiens
6	F (G)	DNA (43-MER)	polymer	43	13035.3	1		Homo sapiens
7	G, I (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
8	H, J (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A: 1 - 1233 (UniProt: Q14683)

PDB	External Database	Details
Gln 1157	Glu 1157	engineered mutation

B: 1 - 1217 (UniProt: Q9UQE7)

PDB	External Database	Details
Gln 1144	Glu 1144	engineered mutation

C: 1 - 631 (UniProt: O60216)

PDB	External Database	Details
Ala 172	Arg 172	engineered mutation
Ala 279	Asp 279	engineered mutation
Ala 450	Arg 450	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	570047.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1061.0
All*	Total formula weight	571108.3

*Water molecules are not included.