Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6WGE

Cryo-EM structure of human Cohesin-NIPBL-DNA complex without STAG1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000070	biological_process	mitotic sister chromatid segregation
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000794	cellular_component	condensed nuclear chromosome
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0007062	biological_process	sister chromatid cohesion
A	0007064	biological_process	mitotic sister chromatid cohesion
A	0008278	cellular_component	cohesin complex
A	0009314	biological_process	response to radiation
A	0016363	cellular_component	nuclear matrix
A	0016887	molecular_function	ATP hydrolysis activity
A	0030892	cellular_component	mitotic cohesin complex
A	0030893	cellular_component	meiotic cohesin complex
A	0034087	biological_process	establishment of mitotic sister chromatid cohesion
A	0034089	biological_process	establishment of meiotic sister chromatid cohesion
A	0035019	biological_process	somatic stem cell population maintenance
A	0036033	molecular_function	mediator complex binding
A	0046982	molecular_function	protein heterodimerization activity
A	0051276	biological_process	chromosome organization
A	0051301	biological_process	cell division
A	0051321	biological_process	meiotic cell cycle
A	0072423	biological_process	response to DNA damage checkpoint signaling
A	0090307	biological_process	mitotic spindle assembly
A	0097431	cellular_component	mitotic spindle pole
B	0000278	biological_process	mitotic cell cycle
B	0000775	cellular_component	chromosome, centromeric region
B	0000785	cellular_component	chromatin
B	0000800	cellular_component	lateral element
B	0003682	molecular_function	chromatin binding
B	0003690	molecular_function	double-stranded DNA binding
B	0003777	molecular_function	microtubule motor activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0005829	cellular_component	cytosol
B	0006275	biological_process	regulation of DNA replication
B	0006281	biological_process	DNA repair
B	0007062	biological_process	sister chromatid cohesion
B	0007064	biological_process	mitotic sister chromatid cohesion
B	0008278	cellular_component	cohesin complex
B	0016363	cellular_component	nuclear matrix
B	0016887	molecular_function	ATP hydrolysis activity
B	0019827	biological_process	stem cell population maintenance
B	0030892	cellular_component	mitotic cohesin complex
B	0030893	cellular_component	meiotic cohesin complex
B	0034087	biological_process	establishment of mitotic sister chromatid cohesion
B	0034089	biological_process	establishment of meiotic sister chromatid cohesion
B	0036033	molecular_function	mediator complex binding
B	0046982	molecular_function	protein heterodimerization activity
B	0048487	molecular_function	beta-tubulin binding
B	0051276	biological_process	chromosome organization
B	0051301	biological_process	cell division
B	0051321	biological_process	meiotic cell cycle
B	0070840	molecular_function	dynein complex binding
B	0090307	biological_process	mitotic spindle assembly
B	0097431	cellular_component	mitotic spindle pole
C	0000775	cellular_component	chromosome, centromeric region
C	0000785	cellular_component	chromatin
C	0000794	cellular_component	condensed nuclear chromosome
C	0000922	cellular_component	spindle pole
C	0001666	biological_process	response to hypoxia
C	0003677	molecular_function	DNA binding
C	0003682	molecular_function	chromatin binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0006281	biological_process	DNA repair
C	0006302	biological_process	double-strand break repair
C	0006310	biological_process	DNA recombination
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0006915	biological_process	apoptotic process
C	0007059	biological_process	chromosome segregation
C	0007062	biological_process	sister chromatid cohesion
C	0007131	biological_process	reciprocal meiotic recombination
C	0008278	cellular_component	cohesin complex
C	0010628	biological_process	positive regulation of gene expression
C	0010629	biological_process	negative regulation of gene expression
C	0010972	biological_process	negative regulation of G2/M transition of mitotic cell cycle
C	0016020	cellular_component	membrane
C	0016363	cellular_component	nuclear matrix
C	0030496	cellular_component	midbody
C	0030892	cellular_component	mitotic cohesin complex
C	0030893	cellular_component	meiotic cohesin complex
C	0032691	biological_process	negative regulation of interleukin-1 beta production
C	0032720	biological_process	negative regulation of tumor necrosis factor production
C	0032733	biological_process	positive regulation of interleukin-10 production
C	0034087	biological_process	establishment of mitotic sister chromatid cohesion
C	0034089	biological_process	establishment of meiotic sister chromatid cohesion
C	0034351	biological_process	negative regulation of glial cell apoptotic process
C	0043524	biological_process	negative regulation of neuron apoptotic process
C	0045841	biological_process	negative regulation of mitotic metaphase/anaphase transition
C	0045876	biological_process	positive regulation of sister chromatid cohesion
C	0051301	biological_process	cell division
C	0071168	biological_process	protein localization to chromatin
C	0106222	molecular_function	lncRNA binding
C	0140297	molecular_function	DNA-binding transcription factor binding
C	0140588	biological_process	chromatin looping
C	1990414	biological_process	replication-born double-strand break repair via sister chromatid exchange
E	0003682	molecular_function	chromatin binding
E	0010468	biological_process	regulation of gene expression
E	0061780	biological_process	mitotic cohesin loading

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue ANP A 2000

Chain	Residue
A	LYS13
A	ASP63
A	GLY67
A	PRO69
A	CYS1210
A	VAL1211
A	MG2001
B	GLN1114
B	LEU1115
B	SER1116
A	SER14
A	ASN34
A	GLY35
A	GLY37
A	LYS38
A	SER39
A	ASN40
A	ARG57

site_id	AC2
Number of Residues	3
Details	binding site for residue MG A 2001

Chain	Residue
A	SER39
A	GLN137
A	ANP2000

site_id	AC3
Number of Residues	17
Details	binding site for residue ANP B 2000

Chain	Residue
A	LYS1120
A	ARG1123
A	ASN1127
A	SER1129
A	GLY1130
A	GLY1131
B	ARG12
B	ASN34
B	GLY35
B	GLY37
B	LYS38
B	SER39
B	ASN40
B	ALA63
B	LEU65
B	GLN141
B	MG2001

site_id	AC4
Number of Residues	3
Details	binding site for residue MG B 2001

Chain	Residue
B	SER39
B	GLN141
B	ANP2000

Functional Information from PROSITE/UniProt

site_id	PS00211
Number of Residues	15
Details	ABC_TRANSPORTER_1 ABC transporters family signature. LSGGQKSLVALALIF

Chain	Residue	Details
B	LEU1115-PHE1129
A	LEU1128-PHE1142

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
E	THR1189
C	ALA450

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
E	SER1197
E	SER2652

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q6KCD5

Chain	Residue	Details
E	SER2493
B	LYS1190

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:21406692

Chain	Residue	Details
E	SER2509
E	SER2511
E	SER2513
E	SER2515

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:16964243, ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:18691976, ECO:0007744\|PubMed:19369195, ECO:0007744\|PubMed:19690332, ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
E	SER2658

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17081983, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
E	THR2667

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
E	SER2672
C	THR623

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	SER454
C	SER545

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
C	LYS48
C	LYS216
C	LYS418

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P97690

Chain	Residue	Details
B	SER1074

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:21406692

Chain	Residue	Details
B	SER1083

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)