6WGE
Cryo-EM structure of human Cohesin-NIPBL-DNA complex without STAG1
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.9 |
Number of Particle | 510507 |
Software | RELION,PHENIX,Gctf,UCSF Chimera |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |