6U1N

GPCR-Beta arrestin structure in lipid bilayer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Muscarinic acetylcholine receptor M2, Vasopressin V2 receptor chimera	polymer	509	56616.2	1	UniProt (P08172) UniProt (P30518) Pfam (PF00001)	Homo sapiens (Human)	V2R, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor
2	B (C)	Beta-arrestin-1	polymer	401	45017.2	1	UniProt (P29066) Pfam (PF00339) Pfam (PF02752)	Rattus norvegicus (Rat)	Arrestin beta-1
3	C (H)	Fab30 heavy chain	polymer	237	25512.4	1	UniProt (V9HW68) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
4	D (L)	Fab30 light chain	polymer	215	23435.1	1	UniProt (Q7Z3Y4) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
5	E (R)	3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide	non-polymer		437.9	1	Chemie (2CU)

Sequence modifications

R: 2 - 466 (UniProt: P08172)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Leu 467	-	linker
Pro 468	-	linker
Glu 469	-	linker
Thr 470	-	linker
Gly 471	-	linker
Gly 472	-	linker
Gly 473	-	linker

R: 474 - 502 (UniProt: P30518)
C: 2 - 393 (UniProt: P29066)

PDB	External Database	Details
Gly -7	-	expression tag
Ser -6	-	expression tag
Pro -5	-	expression tag
Glu -4	-	expression tag
Phe -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Leu 1	-	expression tag
Val 59	Cys 59	engineered mutation
Ser 125	Cys 125	engineered mutation
Leu 140	Cys 140	engineered mutation
Val 150	Cys 150	engineered mutation
Val 242	Cys 242	engineered mutation
Val 251	Cys 251	engineered mutation
Ser 269	Cys 269	engineered mutation

H: 1 - 112 (PDB: 6U1N)
H: 113 - 230 (UniProt: V9HW68)

PDB	External Database	Details
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag

L: 1 - 105 (PDB: 6U1N)
L: 106 - 215 (UniProt: Q7Z3Y4)

PDB	External Database	Details
Ser 124	Glu 145	variant

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	150580.8
Non-Polymers*	Number of molecules	1
	Total formula weight	437.9
All*	Total formula weight	151018.7

*Water molecules are not included.