6T7F

RCR E3 ligase E2-Ubiquitin transthiolation intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase MYCBP2	polymer	261	29410.9	1	UniProt (O75592)	Homo sapiens (Human)	Myc-binding protein 2,Protein associated with Myc
2	B (B)	Ubiquitin-conjugating enzyme E2 D3	polymer	150	16895.2	1	UniProt (P61077) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-conjugating enzyme E2(17)KB 3,Ubiquitin-conjugating enzyme E2-17 kDa 3,Ubiquitin-protein ligase D3
3	C (C)	Polyubiquitin-C	polymer	73	8305.5	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (Human)
4	D, E, F, G, H... (A)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
5	J (B)	3,3-bis(sulfanyl)-~{N}-(1~{H}-1,2,3-triazol-4-ylmethyl)propanamide	non-polymer		218.3	1	Chemie (LWZ)
6	K, L, M (A, B, C)	water	water		18.0	41	Chemie (HOH)

Sequence modifications

A: 4385 - 4640 (UniProt: O75592)

PDB	External Database	Details
Gly 4380	-	expression tag
Pro 4381	-	expression tag
Leu 4382	-	expression tag
Gly 4383	-	expression tag
Ser 4384	-	expression tag

B: 2 - 147 (UniProt: P61077)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	54611.6
Non-Polymers*	Number of molecules	7
	Total formula weight	610.8
All*	Total formula weight	55222.3

*Water molecules are not included.