Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6T7F

RCR E3 ligase E2-Ubiquitin transthiolation intermediate

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000151	cellular_component	ubiquitin ligase complex
B	0000209	biological_process	protein polyubiquitination
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005768	cellular_component	endosome
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006281	biological_process	DNA repair
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006513	biological_process	protein monoubiquitination
B	0006915	biological_process	apoptotic process
B	0010008	cellular_component	endosome membrane
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0019787	molecular_function	ubiquitin-like protein transferase activity
B	0030514	biological_process	negative regulation of BMP signaling pathway
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0036211	biological_process	protein modification process
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051865	biological_process	protein autoubiquitination
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0061631	molecular_function	ubiquitin conjugating enzyme activity
B	0070062	cellular_component	extracellular exosome
B	0070936	biological_process	protein K48-linked ubiquitination
B	0070979	biological_process	protein K11-linked ubiquitination
B	0085020	biological_process	protein K6-linked ubiquitination
B	1903955	biological_process	positive regulation of protein targeting to mitochondrion

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 4701

Chain	Residue
A	CYS4390
A	CYS4393
A	HIS4413
A	CYS4416

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 4702

Chain	Residue
A	CYS4408
A	HIS4410
A	CYS4437
A	CYS4440

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 4703

Chain	Residue
A	CYS4509
A	CYS4537
A	CYS4540
A	CYS4506

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN A 4704

Chain	Residue
A	CYS4549
A	HIS4552
A	CYS4631
A	CYS4634

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN A 4705

Chain	Residue
A	CYS4561
A	CYS4564
A	CYS4579
A	CYS4582

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN A 4706

Chain	Residue
A	CYS4565
A	CYS4600
A	CYS4614
A	HIS4620

site_id	AC7
Number of Residues	6
Details	binding site for residue LWZ B 201

Chain	Residue
A	CYS4520
B	ASN77
B	CYS85
B	ASN114
B	ASP117
C	ARG72

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNInsn.GsICLdiL

Chain	Residue	Details
B	TYR74-LEU89

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
C	LYS27-ASP52

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
C	ARG54
C	ARG72

site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Essential for function

Chain	Residue	Details
C	HIS68
A	CYS4572

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25527291

Chain	Residue	Details
C	SER65
A	CYS4509
A	CYS4537
A	CYS4540
A	CYS4549
A	HIS4552
A	CYS4561
A	CYS4564
A	CYS4565
A	CYS4579
A	CYS4582
A	CYS4393
A	CYS4600
A	CYS4614
A	HIS4620
A	CYS4631
A	CYS4634
A	CYS4408
A	HIS4410
A	HIS4413
A	CYS4416
A	CYS4437
A	CYS4440
A	CYS4506

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457

Chain	Residue	Details
C	THR66
A	PHE4578
A	PHE4586

site_id	SWS_FT_FI5
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
C	LYS6

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
C	LYS63

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
C	LYS11
C	LYS48

site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
C	LYS27

site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
C	LYS29

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
C	LYS33

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)