6SGE
Crystal structure of Human RHOB-GTP in complex with nanobody B6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Rho-related GTP-binding protein RhoB | polymer | 183 | 20814.8 | 2 | UniProt (P62745) Pfam (PF00071) | Homo sapiens (Human) | Rho cDNA clone 6,h6 |
| 2 | B, D (B, D) | Nanobody B6 | polymer | 134 | 14704.3 | 2 | UniProt (A0A4E0W6L3) Pfam (PF07686) | Homo sapiens (Human) | |
| 3 | E, H (A, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 4 | F, G, I, J (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 678 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 183 (UniProt: P62745)
B, D: 2 - 128 (UniProt: A0A4E0W6L3)
| PDB | External Database | Details |
|---|---|---|
| Leu 63 | Gln 63 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 1 | - | expression tag |
| Gln 5 | Leu 7 | conflict |
| Ala 6 | Glu 8 | conflict |
| Phe 11 | Leu 13 | conflict |
| Tyr 27 | Val 29 | conflict |
| Gly 28 | Arg 30 | conflict |
| Ser 29 | Phe 31 | conflict |
| Thr 30 | Ser 32 | conflict |
| Ile 31 | Asn 33 | conflict |
| Thr 33 | Val 35 | conflict |
| Gly 35 | Ala 37 | conflict |
| Phe 37 | Val 39 | conflict |
| Glu 44 | Gly 46 | conflict |
| Arg 45 | Leu 47 | conflict |
| Phe 47 | Trp 49 | conflict |
| Ser 49 | - | insertion |
| Ala 50 | Gly 51 | conflict |
| Ser 52 | Glu 53 | conflict |
| Arg 53 | Lys 54 | conflict |
| Ala 54 | Met 55 | conflict |
| Pro 55 | Asn 56 | conflict |
| Gly 56 | Asp 57 | conflict |
| Pro 57 | Gly 58 | conflict |
| Gln 59 | Thr 60 | conflict |
| Val 80 | Leu 81 | conflict |
| Thr 94 | Val 95 | conflict |
| Pro 99 | Ser 100 | conflict |
| Ile 100 | Trp 101 | conflict |
| Asn 101 | Gly 102 | conflict |
| Asn 102 | His 103 | conflict |
| Arg 103 | Ser 104 | conflict |
| Thr 104 | Arg 105 | conflict |
| Met 105 | Arg 106 | conflict |
| Asp 107 | Gln 108 | conflict |
| Met 109 | Lys 110 | conflict |
| Leu 111 | - | insertion |
| Trp 112 | - | insertion |
| Tyr 114 | Ser 113 | conflict |
| Gln 120 | Leu 119 | conflict |
| Glu 129 | - | expression tag |
| Asn 130 | - | expression tag |
| Leu 131 | - | expression tag |
| Tyr 132 | - | expression tag |
| Phe 133 | - | expression tag |
| Gln 134 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 71038.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1143.6 | |
| All* | Total formula weight | 72181.8 |
