6SGE

Crystal structure of Human RHOB-GTP in complex with nanobody B6

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000281	biological_process	mitotic cytokinesis
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005769	cellular_component	early endosome
A	0005770	cellular_component	late endosome
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0006915	biological_process	apoptotic process
A	0007015	biological_process	actin filament organization
A	0007155	biological_process	cell adhesion
A	0007264	biological_process	small GTPase-mediated signal transduction
A	0007266	biological_process	Rho protein signal transduction
A	0008333	biological_process	endosome to lysosome transport
A	0010008	cellular_component	endosome membrane
A	0010595	biological_process	positive regulation of endothelial cell migration
A	0016020	cellular_component	membrane
A	0019003	molecular_function	GDP binding
A	0030334	biological_process	regulation of cell migration
A	0030335	biological_process	positive regulation of cell migration
A	0030336	biological_process	negative regulation of cell migration
A	0031902	cellular_component	late endosome membrane
A	0032154	cellular_component	cleavage furrow
A	0043065	biological_process	positive regulation of apoptotic process
A	0045202	cellular_component	synapse
A	0045766	biological_process	positive regulation of angiogenesis
A	0045786	biological_process	negative regulation of cell cycle
A	0061154	biological_process	endothelial tube morphogenesis
A	0070062	cellular_component	extracellular exosome
A	0070301	biological_process	cellular response to hydrogen peroxide
A	0071479	biological_process	cellular response to ionizing radiation
A	0098685	cellular_component	Schaffer collateral - CA1 synapse
A	0099159	biological_process	regulation of modification of postsynaptic structure
A	1903141	biological_process	negative regulation of establishment of endothelial barrier
C	0000281	biological_process	mitotic cytokinesis
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005634	cellular_component	nucleus
C	0005769	cellular_component	early endosome
C	0005770	cellular_component	late endosome
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0006915	biological_process	apoptotic process
C	0007015	biological_process	actin filament organization
C	0007155	biological_process	cell adhesion
C	0007264	biological_process	small GTPase-mediated signal transduction
C	0007266	biological_process	Rho protein signal transduction
C	0008333	biological_process	endosome to lysosome transport
C	0010008	cellular_component	endosome membrane
C	0010595	biological_process	positive regulation of endothelial cell migration
C	0016020	cellular_component	membrane
C	0019003	molecular_function	GDP binding
C	0030334	biological_process	regulation of cell migration
C	0030335	biological_process	positive regulation of cell migration
C	0030336	biological_process	negative regulation of cell migration
C	0031902	cellular_component	late endosome membrane
C	0032154	cellular_component	cleavage furrow
C	0043065	biological_process	positive regulation of apoptotic process
C	0045202	cellular_component	synapse
C	0045766	biological_process	positive regulation of angiogenesis
C	0045786	biological_process	negative regulation of cell cycle
C	0061154	biological_process	endothelial tube morphogenesis
C	0070062	cellular_component	extracellular exosome
C	0070301	biological_process	cellular response to hydrogen peroxide
C	0071479	biological_process	cellular response to ionizing radiation
C	0098685	cellular_component	Schaffer collateral - CA1 synapse
C	0099159	biological_process	regulation of modification of postsynaptic structure
C	1903141	biological_process	negative regulation of establishment of endothelial barrier

Functional Information from PDB Data

site_id	AC1
Number of Residues	29
Details	binding site for residue GTP A 201

Chain	Residue
A	GLY14
A	PRO36
A	THR37
A	GLY62
A	LYS118
A	ASP120
A	LEU121
A	SER160
A	ALA161
A	LYS162
A	MG202
A	ALA15
A	HOH316
A	HOH329
A	HOH353
A	HOH362
A	HOH376
A	HOH408
A	HOH422
A	HOH440
A	HOH460
B	ASN113
A	CYS16
A	GLY17
A	LYS18
A	THR19
A	CYS20
A	PHE30
A	TYR34

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 202

Chain	Residue
A	THR19
A	THR37
A	GTP201
A	HOH316
A	HOH362

---

site_id	AC3
Number of Residues	5
Details	binding site for residue MG A 203

Chain	Residue
A	GLU40
A	TRP58
A	ASP59
A	SER73
D	TYR60

---

site_id	AC4
Number of Residues	29
Details	binding site for residue GTP C 201

Chain	Residue
C	GLY14
C	ALA15
C	CYS16
C	GLY17
C	LYS18
C	THR19
C	CYS20
C	PHE30
C	TYR34
C	PRO36
C	THR37
C	GLY62
C	LYS118
C	ASP120
C	LEU121
C	SER160
C	ALA161
C	LYS162
C	MG202
C	HOH303
C	HOH312
C	HOH315
C	HOH345
C	HOH378
C	HOH379
C	HOH387
C	HOH427
C	HOH449
D	ASN113

---

site_id	AC5
Number of Residues	5
Details	binding site for residue MG C 202

Chain	Residue
C	THR19
C	THR37
C	GTP201
C	HOH315
C	HOH345

---

site_id	AC6
Number of Residues	5
Details	binding site for residue MG C 203

Chain	Residue
C	VAL38
C	THR60
C	ALA61
C	HOH326
C	HOH372

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"31522999","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6HXU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6SGE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"31522999","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6SGE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JAN-2006","submissionDatabase":"PDB data bank","title":"The crystal structure of RhoB in the GDP-bound state.","authors":["Turnbull A.P.","Soundararajan M.","Smee C.","Johansson C.","Schoch G.","Gorrec F.","Bray J.","Papagrigoriou E.","von Delft F.","Weigelt J.","Edwards A.","Arrowsmith C.","Sundstrom M.","Doyle D."]}},{"source":"PDB","id":"6HXU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6SGE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"ADP-ribosylasparagine; by botulinum toxin","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P62746","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Glycosylation: {"description":"O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB","evidences":[{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	5
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"29358211","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---