6P08

Ternary structure of the E52D mutant of ANT-4'' with Neomycin, AMP and Pyrophosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Kanamycin nucleotidyltransferase	polymer	256	29121.9	2	UniProt (P05058) Pfam (PF07827) In PDB	Bacillus sp.
2	A	NEOMYCIN	non-polymer		614.6	2	Chemie (NMY)
3	A, D	PYROPHOSPHATE	non-polymer		178.0	2	Chemie (PPV)
4	A, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	A	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
6		water	water		18.0	228	Chemie (HOH)

Sequence modifications

A, D: 1 - 253 (UniProt: P05058)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 52	Glu 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58243.7
Non-Polymers*	Number of molecules	10
	Total formula weight	2376.9
All*	Total formula weight	60620.6

*Water molecules are not included.