6MM7

Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	cAMP-dependent protein kinase catalytic subunit alpha	polymer	339	39598.2	2	UniProt (P05132) Pfam (PF00069)	Mus musculus (Mouse)	PKA C-alpha
2	B, D (C, E)	Ryanodine receptor 2	polymer	209	24285.6	2	UniProt (E9Q401) Pfam (PF02026)	Mus musculus (Mouse)	RyR2,Cardiac muscle ryanodine receptor,Cardiac muscle ryanodine receptor-calcium release channel,Type 2 ryanodine receptor
3	E, F, H, I, M... (A, D)	FORMIC ACID	non-polymer		46.0	8	Chemie (FMT)
4	G (A)	ACETIC ACID	non-polymer		60.1	1	Chemie (ACY)
5	J, Q (A, D)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
6	K, L, S, T, U... (A, C, D, E)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
7	R, W (D, E)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	AA, BA, Y, Z (D, E, A, C)	water	water		18.0	830	Chemie (HOH)

Sequence modifications

A, D: 15 - 350 (UniProt: P05132)

PDB	External Database	Details
Ser 12	-	expression tag
Asn 13	-	expression tag
Ala 14	-	expression tag

C, E: 2699 - 2904 (UniProt: E9Q401)

PDB	External Database	Details
Ser 2696	-	expression tag
Asn 2697	-	expression tag
Ala 2698	-	expression tag
Asp 2813	Ser 2813	engineered mutation
Ala 2879	Lys 2879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127767.6
Non-Polymers*	Number of molecules	20
	Total formula weight	1946.0
All*	Total formula weight	129713.6

*Water molecules are not included.