6MM7
Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.337, 67.560, 176.852 |
| Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
| Resolution | 33.800 - 1.850 |
| R-factor | 0.2134 |
| Rwork | 0.212 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mm6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 4.560 | 1.850 |
| Rmerge | 0.066 | 0.043 | 0.793 |
| Rmeas | 0.071 | 0.047 | 0.859 |
| Rpim | 0.027 | 0.017 | 0.326 |
| Total number of observations | 698657 | ||
| Number of reflections | 111231 | 8082 | 7214 |
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 94.4 | 99.8 | 91.8 |
| Redundancy | 6.3 | 7.3 | 6.4 |
| CC(1/2) | 0.998 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M magnesium formate and 25% (w/v) PEG 3350; 0.05 M HEPES, and 25% ethylene glycol |
