6MM7
Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 58.337, 67.560, 176.852 |
Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
Resolution | 33.800 - 1.850 |
R-factor | 0.2134 |
Rwork | 0.212 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mm6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.376 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.890 |
High resolution limit [Å] | 1.850 | 4.560 | 1.850 |
Rmerge | 0.066 | 0.043 | 0.793 |
Rmeas | 0.071 | 0.047 | 0.859 |
Rpim | 0.027 | 0.017 | 0.326 |
Total number of observations | 698657 | ||
Number of reflections | 111231 | 8082 | 7214 |
<I/σ(I)> | 11.3 | ||
Completeness [%] | 94.4 | 99.8 | 91.8 |
Redundancy | 6.3 | 7.3 | 6.4 |
CC(1/2) | 0.998 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M magnesium formate and 25% (w/v) PEG 3350; 0.05 M HEPES, and 25% ethylene glycol |